A8F597 (SYE1_THELT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Tlet_0765

Organism

Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) [Complete proteome] [HAMAP]

Taxonomic identifier

416591 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367788
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	250 – 254	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	253	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8F597 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 9C0343F255E6FF11	FASTA	470	54,007
	10 20 30 40 50 60 MVRVRFAPSP TGYLHVGGAR TALFNYLFAK HHGGKFILRI EDTDIARSEG IFEENLMKTL 70 80 90 100 110 120 QWLGLNWDEG PDIGGSFGPY RQSERLNIYE EYAKKLIALD KAYEVFAYPE EIEEIREKLL 130 140 150 160 170 180 SKGLTPHYDR TIFEPFATKE RKREYEEKGL KPAIYFSMPR KAFIHNDLVK GTVQFSEGSV 190 200 210 220 230 240 GDFAILRSNG IPTYNFACVV DDMLMQITHV IRGDDHLPNT VKQLALYEAF SARPPEIGHV 250 260 270 280 290 300 STILGPDGKK LSKRHGATSI EELRERGYLP QAVVNYLALL GWSSPDAKEI MSMQEMIERF 310 320 330 340 350 360 SIDRLSKNPA IFDPAKLSWM NGQYIRSTNE EELEQLLKPL LEKWNFIPRN QDWLRRVIIA 370 380 390 400 410 420 VKDRLHTLED FQHVADFFFV KPEIKTETEY QIKCAMLECA DKLESLDRSD KNSIVEVFRS 430 440 450 460 470 TIKKQKVSAK EFYSTLRYVL TGKHEGPELV DIVFLLGPNE VAARIKSILG

References

[1]

"Complete sequence of Thermotoga lettingae TMO." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Bruce D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-301 / DSM 14385 / TMO.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000812 Genomic DNA. Translation: ABV33331.1.
RefSeq	YP_001470395.1. NC_009828.1.
3D structure databases
ProteinModelPortal	A8F597.
SMR	A8F597. Positions 1-470.
ModBase	Search...
Protein-protein interaction databases
STRING	A8F597.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5607932.
GenomeReviews	Gene locus Tlet_0765 in contig CP000812_GR.
KEGG	tle:Tlet_0765.
PATRIC	23932073. VBITheLet125395_0797.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	LMEITHI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	TLET416591:TLET_0765-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_THELT

Accession

Primary (citable) accession number: A8F597

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program