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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi27 – 271ZincUniRule annotation
Metal bindingi207 – 2071ZincUniRule annotation
Metal bindingi232 – 2321ZincUniRule annotation
Metal bindingi236 – 2361ZincUniRule annotation
Binding sitei267 – 2671ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTLET416591:GI64-780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligaseUniRule annotation (EC:6.1.1.16UniRule annotation)
Alternative name(s):
Cysteinyl-tRNA synthetaseUniRule annotation
Short name:
CysRSUniRule annotation
Gene namesi
Name:cysSUniRule annotation
Ordered Locus Names:Tlet_0764
OrganismiPseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)
Taxonomic identifieri416591 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaePseudothermotoga
ProteomesiUP000002016 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Cysteine--tRNA ligasePRO_1000090879Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi416591.Tlet_0764.

Structurei

3D structure databases

ProteinModelPortaliA8F596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi29 – 3911"HIGH" regionAdd
BLAST
Motifi264 – 2685"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiMANGCAY.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

A8F596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFYNTLSKH IEEFKENEPG IVRMYVCGPT VYGLIHIGNA RPMVVFDALR
60 70 80 90 100
RYLEYRGYRV IMVQNFTDID DKIINKSIEF SVDFKDISET FIAEYWRDSM
110 120 130 140 150
ALGVRAPNFS PKTSDFVPEI INAIQNLIAK NHAYVVDGDV YFSVRSFPRY
160 170 180 190 200
GELSHRSIDE LKAGARIDLN EKKRDPLDFA LWKSAKPGEP YWKSPWGNGR
210 220 230 240 250
PGWHIECSVM STKLLGTTLD IHAGGEDLIF PHHENEKAQS EALTGKTFVK
260 270 280 290 300
YWLHNGMIKY TGDKMSKSTG NIFLLREALK KYGADALKIF FLSKHYRSPI
310 320 330 340 350
EFSEKALSSA AKAATRINET FLRFESEFSF AIPKQDSWIN EKRSIFIDSL
360 370 380 390 400
DDDFNTPRAL ALIFELVNSL NRAMDDGNER DALKIYHLLR REFCPVLGLF
410 420 430 440 450
DLPERADNRS IEELVKILID VRAELRAKKQ YELSDMIRSK LRKVGIELKD
460
MPQGTTYTFI KEV
Length:463
Mass (Da):53,437
Last modified:November 13, 2007 - v1
Checksum:iF578FE9B73F966CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA. Translation: ABV33330.1.
RefSeqiWP_012002811.1. NC_009828.1.

Genome annotation databases

EnsemblBacteriaiABV33330; ABV33330; Tlet_0764.
KEGGitle:Tlet_0764.
PATRICi23932071. VBITheLet125395_0796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA. Translation: ABV33330.1.
RefSeqiWP_012002811.1. NC_009828.1.

3D structure databases

ProteinModelPortaliA8F596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416591.Tlet_0764.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV33330; ABV33330; Tlet_0764.
KEGGitle:Tlet_0764.
PATRICi23932071. VBITheLet125395_0796.

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiMANGCAY.
OrthoDBiEOG6RVFXC.

Enzyme and pathway databases

BioCyciTLET416591:GI64-780-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO.

Entry informationi

Entry nameiSYC_PSELT
AccessioniPrimary (citable) accession number: A8F596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 13, 2007
Last modified: July 22, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.