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A8F465

- SYI_THELT

UniProt

A8F465 - SYI_THELT

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Tlet_0382
Organism
Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity
Metal bindingi880 – 8801Zinc By similarity
Metal bindingi883 – 8831Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi903 – 9031Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTLET416591:GI64-387-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Tlet_0382
OrganismiThermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
Taxonomic identifieri416591 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000002016: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911Isoleucine--tRNA ligaseUniRule annotationPRO_1000070892Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi416591.Tlet_0382.

Structurei

3D structure databases

ProteinModelPortaliA8F465.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8F465-1 [UniParc]FASTAAdd to Basket

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MDYRSTLNLP GTEFSMRANL VEKEPRILEK WQKMNIYDYL LKHRENNPTF    50
VLHDGPPYAN GDIHIGTAMN KILKDIVVRY KTLRGFKSPY VPGWDTHGLP 100
IEHKVTTMLG EKARSMSQTE LRKACEEFAK QQIEMQKKQF QRLGVIGDWE 150
NYYATLNPEY EYKIYEIFAK LVEDGYVYRA LKPVLWCNNC GTALAQAEIE 200
YHDHDSPSIY VKFKMENSEN EYIIIWTTTP WTLPGNTGIA VHPDQQYVKI 250
EVGSEIWILA EKLLKMAMQE IGIDEYRILE KFKGHFLEGK QAVHPIFDRK 300
SRIITADFVD METGTGCVHI APGHGEEDYE FGHLVNGLAV ISPVDEKGYF 350
TEEAGKYSGM HIEKANKQII EDLKNNGSLL KESTIKHSYP HCWRCKKPVI 400
FRATEQWFIS VEKNNLRQRV LENIQNVAWI PAWGKNRISA MVEERPDWCI 450
SRQRAWGTPI PAFRCSKCSE VVLDARLIRH FAEIVKKMGT NAWFELSEKQ 500
LLPDGYVCPH CGSEDLVKMN DTLDVWIDSG SSFEAVLTTR PELTFPADMY 550
LEGSDQHRGW FNSSLVLSVI THDKPPYKTV LTHGFIKDAE GKKMSKSLGN 600
VVDPLEVCSK YGADVLRLWL ASSDYFNDIR ISKNIIFQQV EVYKKIRNTI 650
RYLLGNLQDF SVTDSIGFQK MLPVDRWALG RLQQIIKSVT EAYESYEFSK 700
AYNILVKYCS VELSAIYLDI VKDRLYVEGK HSLKRRSAQT VLYNILKSLL 750
VMFSPILAFT CEEAYEFLNF GEKKYQTVMA ENWPEYKEEY IDTNLMNDFE 800
ELLQLRDLVL KALEESRQNG LIGHSLDAKV AIKTGSKKTL DLLKKYSAYL 850
EEFFIVSKVE ICNSDENLHI EVEHAEGEKC ERCWKYDPLT NSDSEFPNIC 900
PRCMAVLKEK R 911
Length:911
Mass (Da):105,342
Last modified:November 13, 2007 - v1
Checksum:iB50424733D43F09B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000812 Genomic DNA. Translation: ABV32949.1.
RefSeqiYP_001470013.1. NC_009828.1.

Genome annotation databases

EnsemblBacteriaiABV32949; ABV32949; Tlet_0382.
GeneIDi5608531.
KEGGitle:Tlet_0382.
PATRICi23931275. VBITheLet125395_0407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000812 Genomic DNA. Translation: ABV32949.1 .
RefSeqi YP_001470013.1. NC_009828.1.

3D structure databases

ProteinModelPortali A8F465.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416591.Tlet_0382.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV32949 ; ABV32949 ; Tlet_0382 .
GeneIDi 5608531.
KEGGi tle:Tlet_0382.
PATRICi 23931275. VBITheLet125395_0407.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TLET416591:GI64-387-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-301 / DSM 14385 / TMO.

Entry informationi

Entry nameiSYI_THELT
AccessioniPrimary (citable) accession number: A8F465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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