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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi880 – 8801ZincUniRule annotation
Metal bindingi883 – 8831ZincUniRule annotation
Metal bindingi900 – 9001ZincUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTLET416591:GI64-387-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Tlet_0382
OrganismiThermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
Taxonomic identifieri416591 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000002016: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911Isoleucine--tRNA ligasePRO_1000070892Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi416591.Tlet_0382.

Structurei

3D structure databases

ProteinModelPortaliA8F465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8F465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYRSTLNLP GTEFSMRANL VEKEPRILEK WQKMNIYDYL LKHRENNPTF
60 70 80 90 100
VLHDGPPYAN GDIHIGTAMN KILKDIVVRY KTLRGFKSPY VPGWDTHGLP
110 120 130 140 150
IEHKVTTMLG EKARSMSQTE LRKACEEFAK QQIEMQKKQF QRLGVIGDWE
160 170 180 190 200
NYYATLNPEY EYKIYEIFAK LVEDGYVYRA LKPVLWCNNC GTALAQAEIE
210 220 230 240 250
YHDHDSPSIY VKFKMENSEN EYIIIWTTTP WTLPGNTGIA VHPDQQYVKI
260 270 280 290 300
EVGSEIWILA EKLLKMAMQE IGIDEYRILE KFKGHFLEGK QAVHPIFDRK
310 320 330 340 350
SRIITADFVD METGTGCVHI APGHGEEDYE FGHLVNGLAV ISPVDEKGYF
360 370 380 390 400
TEEAGKYSGM HIEKANKQII EDLKNNGSLL KESTIKHSYP HCWRCKKPVI
410 420 430 440 450
FRATEQWFIS VEKNNLRQRV LENIQNVAWI PAWGKNRISA MVEERPDWCI
460 470 480 490 500
SRQRAWGTPI PAFRCSKCSE VVLDARLIRH FAEIVKKMGT NAWFELSEKQ
510 520 530 540 550
LLPDGYVCPH CGSEDLVKMN DTLDVWIDSG SSFEAVLTTR PELTFPADMY
560 570 580 590 600
LEGSDQHRGW FNSSLVLSVI THDKPPYKTV LTHGFIKDAE GKKMSKSLGN
610 620 630 640 650
VVDPLEVCSK YGADVLRLWL ASSDYFNDIR ISKNIIFQQV EVYKKIRNTI
660 670 680 690 700
RYLLGNLQDF SVTDSIGFQK MLPVDRWALG RLQQIIKSVT EAYESYEFSK
710 720 730 740 750
AYNILVKYCS VELSAIYLDI VKDRLYVEGK HSLKRRSAQT VLYNILKSLL
760 770 780 790 800
VMFSPILAFT CEEAYEFLNF GEKKYQTVMA ENWPEYKEEY IDTNLMNDFE
810 820 830 840 850
ELLQLRDLVL KALEESRQNG LIGHSLDAKV AIKTGSKKTL DLLKKYSAYL
860 870 880 890 900
EEFFIVSKVE ICNSDENLHI EVEHAEGEKC ERCWKYDPLT NSDSEFPNIC
910
PRCMAVLKEK R
Length:911
Mass (Da):105,342
Last modified:November 13, 2007 - v1
Checksum:iB50424733D43F09B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA. Translation: ABV32949.1.
RefSeqiYP_001470013.1. NC_009828.1.

Genome annotation databases

EnsemblBacteriaiABV32949; ABV32949; Tlet_0382.
GeneIDi5608531.
KEGGitle:Tlet_0382.
PATRICi23931275. VBITheLet125395_0407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA. Translation: ABV32949.1.
RefSeqiYP_001470013.1. NC_009828.1.

3D structure databases

ProteinModelPortaliA8F465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi416591.Tlet_0382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV32949; ABV32949; Tlet_0382.
GeneIDi5608531.
KEGGitle:Tlet_0382.
PATRICi23931275. VBITheLet125395_0407.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciTLET416591:GI64-387-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-301 / DSM 14385 / TMO.

Entry informationi

Entry nameiSYI_THELT
AccessioniPrimary (citable) accession number: A8F465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: March 4, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.