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Protein

Argininosuccinate synthase

Gene

argG

Organism
Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei84CitrullineUniRule annotation1
Binding sitei114ATP; via amide nitrogenUniRule annotation1
Binding sitei116AspartateUniRule annotation1
Binding sitei120AspartateUniRule annotation1
Binding sitei120CitrullineUniRule annotation1
Binding sitei121AspartateUniRule annotation1
Binding sitei124CitrullineUniRule annotation1
Binding sitei177CitrullineUniRule annotation1
Binding sitei186CitrullineUniRule annotation1
Binding sitei267CitrullineUniRule annotation1
Binding sitei279CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 15ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTLET416591:G1G9T-372-MONOMER
UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Tlet_0363
OrganismiPseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)
Taxonomic identifieri416591 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaePseudothermotoga
Proteomesi
  • UP000002016 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003294741 – 425Argininosuccinate synthaseAdd BLAST425

Proteomic databases

PRIDEiA8F446

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi416591.Tlet_0363

Structurei

3D structure databases

ProteinModelPortaliA8F446
SMRiA8F446
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM
OrthoDBiPOG091H01U0

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.90.1260.10, 1 hit
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

A8F446-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVILAYSG GLDTSVILKW LSEKGFEVIA FVANLGQKED FKAIECKALS
60 70 80 90 100
TGASKVYVED LRREFVTEFV FPALMGNALY EGRYLLGTAL SRPLIAKKQV
110 120 130 140 150
EIAEKENAAF VAHGATGKGN DQVRFELTYA ALNPFLKVLS PWKDIEFLKL
160 170 180 190 200
FKGRTDLLAY AKQKCILTRA TIEKPYSEDE NLMHISHEGG KLEDPLYIAG
210 220 230 240 250
EDVFSWTNSP KNAPDEEVFL ELHFRSGLPV KVVNLNDGST KEDPLELFEY
260 270 280 290 300
LNRIGSKYGV GRVDMVENRF IGIKSRGIYE TPGATILWIA HRDLEGIAMD
310 320 330 340 350
KEVMHLRDML SVKFSELIYN GFWFSPEMDF LLSAFRKSQE GIEGKVNLII
360 370 380 390 400
YKGNVVPVSR FSPTSLYDQS LSSMDVDGGF DATDSKGFIN IHALRLRAHN
410 420
LVLRSRDPFG WRRGIIDAEI MGKGV
Length:425
Mass (Da):47,789
Last modified:November 13, 2007 - v1
Checksum:i70CFF50EA000ED6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000812 Genomic DNA Translation: ABV32930.1
RefSeqiWP_012002411.1, NC_009828.1

Genome annotation databases

EnsemblBacteriaiABV32930; ABV32930; Tlet_0363
KEGGitle:Tlet_0363

Similar proteinsi

Entry informationi

Entry nameiASSY_PSELT
AccessioniPrimary (citable) accession number: A8F446
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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