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A8F3S6 (RUVC_THELT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Tlet_0240
OrganismThermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) [Complete proteome] [HAMAP]
Taxonomic identifier416591 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length162 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 162162Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000057269

Sites

Metal binding71Magnesium By similarity
Metal binding671Magnesium By similarity
Metal binding1401Magnesium By similarity
Metal binding1431Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
A8F3S6 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: C8E19634017EF0D8

FASTA16217,868
        10         20         30         40         50         60 
MIIFGVDPGF GILGYGVLSV SGNSFQHVSH GTIQTEKQQN IALRLKVLYE ELSNVIDNFK 

        70         80         90        100        110        120 
PSEIAMEKLF FSRNITTAIS VGEARGIVLL LAAQRNIPVF EYTPHEIKKA VTGSGKASKK 

       130        140        150        160 
DVQQMIKILL NLKELPKPDD AADGLAIAWC HCAVRNITRR FS 

« Hide

References

[1]"Complete sequence of Thermotoga lettingae TMO."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Foster B., Bruce D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-301 / DSM 14385 / TMO.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000812 Genomic DNA. Translation: ABV32810.1.
RefSeqYP_001469874.1. NC_009828.1.

3D structure databases

ProteinModelPortalA8F3S6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416591.Tlet_0240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV32810; ABV32810; Tlet_0240.
GeneID5607881.
KEGGtle:Tlet_0240.
PATRIC23930975. VBITheLet125395_0259.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012182.
KOK01159.
OMARQVQEMV.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycTLET416591:GI64-243-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUVC_THELT
AccessionPrimary (citable) accession number: A8F3S6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families