A8F2Z1 (SYGA_RICM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	RMA_1337

Organism

Rickettsia massiliae (strain Mtu5) [Complete proteome] [HAMAP]

Taxonomic identifier

416276 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia massiliae

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Glycine--tRNA ligase alpha subunit HAMAP-Rule MF_00254		PRO_1000059056

Sequences

	Sequence		Length	Mass (Da)
	A8F2Z1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: CC7BA89CCC2DF1B6	FASTA	288	33,139
	10 20 30 40 50 60 MKKLSFQQII LTLQNYWQDY GCAILQPYDA HVGAGTFHPA TVLRCLGTKP WSVAYVQPSR 70 80 90 100 110 120 RPGDSRYGMH PNRMQHYYQF QVILKPSPDN IQELYLKSLE CLGIDLKIHD IRFVEDDWES 130 140 150 160 170 180 PTLGAAGLGW EVWCNGMEVS QFTYMQQIGG IECRPVAGEI TYGLDRLALY IQGVDEVREL 190 200 210 220 230 240 DWNGQVGEKA LKYGEVDFEA ERQFSKYNLE LADSEMLLRH FKDSEDQCER LIKANLPMPA 250 260 270 280 YDECLKASHA FNQLNALGVI SVTERASYVL RVRHLARICC TKWLEMNK

References

[1]

"Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome." Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D. Genome Res. 17:1657-1664(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mtu5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000683 Genomic DNA. Translation: ABV85277.1.
RefSeq	YP_001499824.1. NC_009900.1.
3D structure databases
ProteinModelPortal	A8F2Z1.
SMR	A8F2Z1. Positions 4-288.
ModBase	Search...
Protein-protein interaction databases
STRING	416276.RMA_1337.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV85277; ABV85277; RMA_1337.
GeneID	5665700.
KEGG	rms:RMA_1337.
PATRIC	17897390. VBIRicMas83254_1655.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0752.
HOGENOM	HOG000264291.
KO	K01878.
OMA	CVILQPY.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	RMAS416276:GJD3-1368-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha.
InterPro	IPR006194. Gly-tRNA-synth_heterodimer. IPR002310. Gly-tRNA_ligase_asu. [Graphical view]
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. glyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_RICM5

Accession

Primary (citable) accession number: A8F2Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program