ID   A8F2Y7_RICM5            Unreviewed;       313 AA.
AC   A8F2Y7;
DT   13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT   13-NOV-2007, sequence version 1.
DT   27-MAR-2024, entry version 84.
DE   RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE            EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN   OrderedLocusNames=RMA_1333 {ECO:0000313|EMBL:ABV85273.1};
OS   Rickettsia massiliae (strain Mtu5).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=416276 {ECO:0000313|EMBL:ABV85273.1, ECO:0000313|Proteomes:UP000001311};
RN   [1] {ECO:0000313|EMBL:ABV85273.1, ECO:0000313|Proteomes:UP000001311}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mtu5 {ECO:0000313|Proteomes:UP000001311};
RX   PubMed=17916642; DOI=10.1101/gr.6742107;
RA   Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D.;
RT   "Lateral gene transfer between obligate intracellular bacteria: evidence
RT   from the Rickettsia massiliae genome.";
RL   Genome Res. 17:1657-1664(2007).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC       {ECO:0000256|RuleBase:RU362028}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000256|RuleBase:RU362028};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC         pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC         Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00036882};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR   EMBL; CP000683; ABV85273.1; -; Genomic_DNA.
DR   RefSeq; WP_012153234.1; NC_009900.1.
DR   AlphaFoldDB; A8F2Y7; -.
DR   KEGG; rms:RMA_1333; -.
DR   HOGENOM; CLU_016902_4_4_5; -.
DR   Proteomes; UP000001311; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR   Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   NCBIfam; TIGR00005; rluA_subfam; 1.
DR   PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR   PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase {ECO:0000256|RuleBase:RU362028};
KW   RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT   DOMAIN          13..78
FT                   /note="RNA-binding S4"
FT                   /evidence="ECO:0000259|SMART:SM00363"
FT   ACT_SITE        137
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ   SEQUENCE   313 AA;  35320 MW;  244F36EFA0F7A431 CRC64;
     MLKYSVPHEL NDIRLDKALS SLLENVSRNQ IQKAIKDSQV LVNDVIISDP DVLVKENDII
     LFSFKEPEEL KIAAANVALD IIYEDDDLIV INKAAGMTVH PGAGHHDDTL VNALLHHTKN
     LSDIGSSERP GIVHRLDKDT TGLMVVAKNN KAHMLLANQI EQRQVIRKYK VLVWGVINPL
     EGVIKNNIGR SRSDRQKMTI LKYGGKEAVT HYKTLELFYK GSISMVECKL STGRTHQIRV
     QLSHLKHSVV GDQTYGNNDR KIAHSPPELK AKLIDFKRQA LHSWYLSFTH PTSNEIMAFS
     CKLPRDMEEI ISL
//