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A8F2M4

- LIPA_RICM5

UniProt

A8F2M4 - LIPA_RICM5

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Protein

Lipoyl synthase

Gene
lipA, RMA_1171
Organism
Rickettsia massiliae (strain Mtu5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi42 – 421Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi48 – 481Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi63 – 631Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi70 – 701Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRMAS416276:GJD3-1199-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:RMA_1171
OrganismiRickettsia massiliae (strain Mtu5)
Taxonomic identifieri416276 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000001311: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Lipoyl synthaseUniRule annotationPRO_1000058578Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi416276.RMA_1171.

Structurei

3D structure databases

ProteinModelPortaliA8F2M4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8F2M4-1 [UniParc]FASTAAdd to Basket

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MANLNKRPDW IKVKAPNSTE YYNTKDLIKN LRLNTVCEEA ACPNIGECWS    50
KKHTTVMILG SVCTRACRFC NVKTGRPDLL DPHEPQRLAE AVQKLNLKHV 100
VITSVDRDDL EDGGASHFAE CISEIRKSSP NTTIEILTPD FLRKEGAAEI 150
IANAKPDVFN HNVETVPSLY KTIRPGARYY NSLSLLHNIK KLSPDIFTKS 200
GMMVGLGEEI NEVVQVMDDL REAKVDFLTI GQYLQPTKNH AEVAKYVTPE 250
EFKYLERVAK TKGFLMVSAS PLTRSSYHAD EDFQKLKENY QQKLVS 296
Length:296
Mass (Da):33,460
Last modified:November 13, 2007 - v1
Checksum:i1F91449DD5555BF8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000683 Genomic DNA. Translation: ABV85160.1.
RefSeqiYP_001499707.1. NC_009900.1.

Genome annotation databases

EnsemblBacteriaiABV85160; ABV85160; RMA_1171.
GeneIDi5665156.
KEGGirms:RMA_1171.
PATRICi17896971. VBIRicMas83254_1449.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000683 Genomic DNA. Translation: ABV85160.1 .
RefSeqi YP_001499707.1. NC_009900.1.

3D structure databases

ProteinModelPortali A8F2M4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 416276.RMA_1171.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV85160 ; ABV85160 ; RMA_1171 .
GeneIDi 5665156.
KEGGi rms:RMA_1171.
PATRICi 17896971. VBIRicMas83254_1449.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci RMAS416276:GJD3-1199-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome."
    Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D.
    Genome Res. 17:1657-1664(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mtu5.

Entry informationi

Entry nameiLIPA_RICM5
AccessioniPrimary (citable) accession number: A8F2M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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