Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8F110 (DDL_RICM5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:RMA_0341
OrganismRickettsia massiliae (strain Mtu5) [Complete proteome] [HAMAP]
Taxonomic identifier416276 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 321321D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000057329

Regions

Domain121 – 315195ATP-grasp
Nucleotide binding147 – 19953ATP By similarity

Sites

Metal binding2681Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 2 By similarity
Metal binding2841Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8F110 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 3DD68EED3FE3EA73

FASTA32135,920
        10         20         30         40         50         60 
MHKYQTHWVE HSIVKILSSA GKKYIALMAG GMSAEREVSL VSSEGVSKAL IELGYSVTFI 

        70         80         90        100        110        120 
DMGADITVRL QEIKPDIVFN CLHGTYGEDG CLPGLLNIMR IPYTHSGMLS SALAFNKIHS 

       130        140        150        160        170        180 
RSWFLTNNIN MAESIVVNKS DNIKNDPMKR PYVIKPLTQG SSIGVEVIFA EDNFNFADYD 

       190        200        210        220        230        240 
FPYGDQVIIE QYIKGRELQV AVLNGKALGV LEIKLLKNRF YDYETKYTEG FADHLCPAPL 

       250        260        270        280        290        300 
PANLYEKLLI ESEKIYKTMN CKGPARAEFI LEEQTNKLYA LELNTHPGMT PLSIVPEIAA 

       310        320 
YAGINFTNLI EEIIKTASFE S 

« Hide

References

[1]"Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome."
Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D.
Genome Res. 17:1657-1664(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Mtu5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000683 Genomic DNA. Translation: ABV84596.1.
RefSeqYP_001499143.1. NC_009900.1.

3D structure databases

ProteinModelPortalA8F110.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416276.RMA_0341.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV84596; ABV84596; RMA_0341.
GeneID5665609.
KEGGrms:RMA_0341.
PATRIC17894889. VBIRicMas83254_0432.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAEDPARES.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycRMAS416276:GJD3-345-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_RICM5
AccessionPrimary (citable) accession number: A8F110
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways