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A8F0M4 (RUVC_RICM5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:RMA_0166
OrganismRickettsia massiliae (strain Mtu5) [Complete proteome] [HAMAP]
Taxonomic identifier416276 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence caution

The sequence ABV84460.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000332437

Sites

Metal binding71Magnesium By similarity
Metal binding671Magnesium By similarity
Metal binding1401Magnesium By similarity
Metal binding1431Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
A8F0M4 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: DE9428D09C8D10F8

FASTA15717,274
        10         20         30         40         50         60 
MIILGIDPAL GSLGWAVVAK ETAQLKYLAS GIIKTNSKDA IHHRLAFINS TLEKVILEYQ 

        70         80         90        100        110        120 
PNMAAIEETF VNTNSVTSLK LGYARGAIMS LIGRYNLDMR EFKPNTVKKT VTGYGHAEKD 

       130        140        150 
QILHMIKLLL PGTFLITNSD EADAVAIAYT CLVTKNY 

« Hide

References

[1]"Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome."
Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D.
Genome Res. 17:1657-1664(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Mtu5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000683 Genomic DNA. Translation: ABV84460.1. Different initiation.
RefSeqYP_001499007.1. NC_009900.1.

3D structure databases

ProteinModelPortalA8F0M4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING416276.RMA_0166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV84460; ABV84460; RMA_0166.
GeneID5664995.
KEGGrms:RMA_0166.
PATRIC17894454. VBIRicMas83254_0220.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycRMAS416276:GJD3-170-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_RICM5
AccessionPrimary (citable) accession number: A8F0M4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families