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A8F0M4

- RUVC_RICM5

UniProt

A8F0M4 - RUVC_RICM5

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Rickettsia massiliae (strain Mtu5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 2 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71MagnesiumUniRule annotation
    Metal bindingi67 – 671MagnesiumUniRule annotation
    Metal bindingi140 – 1401MagnesiumUniRule annotation
    Metal bindingi143 – 1431MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRMAS416276:GJD3-170-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:RMA_0166
    OrganismiRickettsia massiliae (strain Mtu5)
    Taxonomic identifieri416276 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000001311: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Crossover junction endodeoxyribonuclease RuvCPRO_0000332437Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi416276.RMA_0166.

    Structurei

    3D structure databases

    ProteinModelPortaliA8F0M4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012181.
    KOiK01159.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8F0M4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIILGIDPAL GSLGWAVVAK ETAQLKYLAS GIIKTNSKDA IHHRLAFINS    50
    TLEKVILEYQ PNMAAIEETF VNTNSVTSLK LGYARGAIMS LIGRYNLDMR 100
    EFKPNTVKKT VTGYGHAEKD QILHMIKLLL PGTFLITNSD EADAVAIAYT 150
    CLVTKNY 157
    Length:157
    Mass (Da):17,274
    Last modified:April 29, 2008 - v2
    Checksum:iDE9428D09C8D10F8
    GO

    Sequence cautioni

    The sequence ABV84460.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000683 Genomic DNA. Translation: ABV84460.1. Different initiation.
    RefSeqiYP_001499007.1. NC_009900.1.

    Genome annotation databases

    EnsemblBacteriaiABV84460; ABV84460; RMA_0166.
    GeneIDi5664995.
    KEGGirms:RMA_0166.
    PATRICi17894454. VBIRicMas83254_0220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000683 Genomic DNA. Translation: ABV84460.1 . Different initiation.
    RefSeqi YP_001499007.1. NC_009900.1.

    3D structure databases

    ProteinModelPortali A8F0M4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 416276.RMA_0166.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV84460 ; ABV84460 ; RMA_0166 .
    GeneIDi 5664995.
    KEGGi rms:RMA_0166.
    PATRICi 17894454. VBIRicMas83254_0220.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012181.
    KOi K01159.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci RMAS416276:GJD3-170-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome."
      Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D.
      Genome Res. 17:1657-1664(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mtu5.

    Entry informationi

    Entry nameiRUVC_RICM5
    AccessioniPrimary (citable) accession number: A8F0M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 41 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3