A8F0M1 (LEP_RICM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	RMA_0163

Organism

Rickettsia massiliae (strain Mtu5) [Complete proteome] [HAMAP]

Taxonomic identifier

416276 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.
Sequence caution	The sequence ABV84457.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Signal peptidase I		PRO_0000316276
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Topological domain	42 – 266	225	Periplasmic Potential
Sites
	Active site	45	1	By similarity
	Active site	108	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8F0M1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: CE46F86BB5907FF1	FASTA	266	31,190
	10 20 30 40 50 60 MQTDNTKSNT NKTAKQEWGS FVFVICIALL IRILIMEPFT VPTGSMKATI LENDYIFSTK 70 80 90 100 110 120 YSYGYSNYSL SFFDFIPLFK GRIFAREPER GDIVVFRPPH DMSVRYIKRL IGLPGDKIQL 130 140 150 160 170 180 IDDVIYINDK KIERTEVGTY ISEEGIKYLK FKETLPNGRT YFSYKLAPIY GVIYNDRYGN 190 200 210 220 230 240 TDVFYVPEGK YFFLGDNRDQ SNDSRVNLGF VPFENFIAKA QFIWFSTKIT WWDNDIGVIN 250 260 LVLKLKPWIE SVRLNRIFRN LYNTDV

References

[1]

"Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome." Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D. Genome Res. 17:1657-1664(2007) [PubMed: 17916642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mtu5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000683 Genomic DNA. Translation: ABV84457.1. Different initiation.
RefSeq	YP_001499004.1. NC_009900.1.
3D structure databases
ProteinModelPortal	A8F0M1.
ModBase	Search...
Protein-protein interaction databases
STRING	A8F0M1.
Protein family/group databases
MEROPS	S26.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5666018.
GenomeReviews	Gene locus RMA_0163 in contig CP000683_GR.
KEGG	rms:RMA_0163.
PATRIC	17894448. VBIRicMas83254_0217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0681.
HOGENOM	HBG596607.
OMA	KPWIESV.
ProtClustDB	CLSK870751.
Enzyme and pathway databases
BioCyc	RMAS416276:RMA_0163-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits.
KO	K03100.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICM5

Accession

Primary (citable) accession number: A8F0M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	January 25, 2012
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program