Reviewed, UniProtKB/Swiss-Prot A8F0M0 (NUOF_RICM5)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	RMA_0162

Organism

Rickettsia massiliae (strain Mtu5) [Complete proteome] [HAMAP]

Taxonomic identifier

416276 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	NADH-quinone oxidoreductase subunit F		PRO_0000316287
Regions
	Nucleotide binding	54 – 63	10	NAD By similarity
	Nucleotide binding	166 – 213	48	FMN By similarity
Sites
	Metal binding	344	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	347	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	350	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	390	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8F0M0-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 6FF6C996607F25B8	FASTA	421	46,225
	10 20 30 40 50 60 MLKEEDKIFT NLHGQQSHDL KSSKKRGDWE NTKALLDKGR DFIVEEVKKS GLRGRGGAGF 70 80 90 100 110 120 STGMKWSFMP KNSEKPCYLV VNADESEPGT CKDRDILRFE PHKLIEGCLL ASFAIGANNC 130 140 150 160 170 180 YIYIRGEFYN EASNIQRALD EAYKEGLIGK NACDSGFDCN IYLHRGAGAY ICGEETALLE 190 200 210 220 230 240 SLEGKKGMPR LKPPFPAGFG LYGCPTTINN VESIAVVPTI LRRGASWFAG IGKPNNTGTK 250 260 270 280 290 300 IFCISGHVNK PCNVEEAMGI SLKELIEKYA GGVRGGWDNL KAIIPGGSSV PLLPKLLCEV 310 320 330 340 350 360 EMDFDSLRTA GSGLGTGGII VMDKSTDIIY AIARLSKFYM HESCGQCTPC REGTGWMWRV 370 380 390 400 410 420 MMRLVKGNAK KSEIDELLNV TKEIEGHTIC ALGDAAAWPI QGLIRHFRSE IEARIKSYSV V

References

[1]	"Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome." Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D. Genome Res. 17:1657-1664(2007) [PubMed: 17916642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000683 Genomic DNA. Translation: ABV84456.1.
RefSeq	YP_001499003.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5666017.
GenomeReviews	Gene locus RMA_0162 in contig CP000683_GR.
KEGG	rms:RMA_0162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8F0M0. PCNVEEE.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_RICM5

Accession

Primary (citable) accession number: A8F0M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)