A8F0H9 (QUEF_RICM5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	RMA_0110

Organism

Rickettsia massiliae (strain Mtu5) [Complete proteome] [HAMAP]

Taxonomic identifier

416276 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000062359

Sequences

	Sequence		Length	Mass (Da)
	A8F0H9 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: D666531251CF32C1	FASTA	273	31,119
	10 20 30 40 50 60 MPLSTSLLGK KSTYKDSYDV TLLFKIPRIN NRNELGINSN NLPFYGVDVW NTYELSCLNK 70 80 90 100 110 120 NGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES VKELERIILQ DLSNVTHAKV 130 140 150 160 170 180 TGRIFPINTK VEFGVPSGKN IDDLDIVCNN YGAPDNSLIE YEDVLVEEEI NSNLLKSNCL 190 200 210 220 230 240 VTGQPDWGTI VIKYKGKKLK HDSFLKYLIS FRNCNEFAEQ CAERIFTDIK NAISPDFLSI 250 260 270 SIVYTRRGGI DICPYRSTDK SYTLPSDKRF IRQ

References

[1]

"Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genome." Blanc G., Ogata H., Robert C., Audic S., Claverie J.-M., Raoult D. Genome Res. 17:1657-1664(2007) [PubMed: 17916642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mtu5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000683 Genomic DNA. Translation: ABV84415.1.
RefSeq	YP_001498962.1. NC_009900.1.
3D structure databases
ProteinModelPortal	A8F0H9.
ModBase	Search...
Protein-protein interaction databases
STRING	A8F0H9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5665727.
GenomeReviews	Gene locus RMA_0110 in contig CP000683_GR.
KEGG	rms:RMA_0110.
PATRIC	17894322. VBIRicMas83254_0156.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
OMA	CVERIFC.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	RMAS416276:RMA_0110-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RICM5

Accession

Primary (citable) accession number: A8F0H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program