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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Rickettsia massiliae (strain Mtu5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei179 – 1791Thioimide intermediateUniRule annotation
Active sitei186 – 1861Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 842NADPHUniRule annotation
Nucleotide bindingi247 – 2482NADPHUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciRMAS416276:GJD3-112-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:RMA_0110
OrganismiRickettsia massiliae (strain Mtu5)
Taxonomic identifieri416276 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000001311 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_1000062359Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 833Substrate bindingUniRule annotation
Regioni218 – 2192Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

A8F0H9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLSTSLLGK KSTYKDSYDV TLLFKIPRIN NRNELGINSN NLPFYGVDVW
60 70 80 90 100
NTYELSCLNK NGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES
110 120 130 140 150
VKELERIILQ DLSNVTHAKV TGRIFPINTK VEFGVPSGKN IDDLDIVCNN
160 170 180 190 200
YGAPDNSLIE YEDVLVEEEI NSNLLKSNCL VTGQPDWGTI VIKYKGKKLK
210 220 230 240 250
HDSFLKYLIS FRNCNEFAEQ CAERIFTDIK NAISPDFLSI SIVYTRRGGI
260 270
DICPYRSTDK SYTLPSDKRF IRQ
Length:273
Mass (Da):31,119
Last modified:November 13, 2007 - v1
Checksum:iD666531251CF32C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000683 Genomic DNA. Translation: ABV84415.1.
RefSeqiWP_012152396.1. NC_009900.1.

Genome annotation databases

EnsemblBacteriaiABV84415; ABV84415; RMA_0110.
KEGGirms:RMA_0110.
PATRICi17894322. VBIRicMas83254_0156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000683 Genomic DNA. Translation: ABV84415.1.
RefSeqiWP_012152396.1. NC_009900.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV84415; ABV84415; RMA_0110.
KEGGirms:RMA_0110.
PATRICi17894322. VBIRicMas83254_0156.

Phylogenomic databases

HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciRMAS416276:GJD3-112-MONOMER.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEF_RICM5
AccessioniPrimary (citable) accession number: A8F0H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.