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A8EZU2

- LIPA_RICCK

UniProt

A8EZU2 - LIPA_RICCK

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Protein
Lipoyl synthase
Gene
lipA, A1E_04770
Organism
Rickettsia canadensis (strain McKiel)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi42 – 421Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi48 – 481Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi63 – 631Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi70 – 701Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRCAN293613:GHI3-950-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:A1E_04770
OrganismiRickettsia canadensis (strain McKiel)
Taxonomic identifieri293613 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000007056: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Lipoyl synthaseUniRule annotation
PRO_1000012267Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi293613.A1E_04770.

Structurei

3D structure databases

ProteinModelPortaliA8EZU2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiFERIATN.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8EZU2-1 [UniParc]FASTAAdd to Basket

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MANLNKRPDW IKVKAPNSVE YYNTKDLIKN LRLNTVCEEA ACPNIGECWS    50
KKHATMMILG SVCTRACRFC NVKTGRPDLL DPHEPQRLAE AVQKLNLKHV 100
VITSVDRDDL EDGGALHFAE CINEIRKSSP NTTVEILTPD FLRKEGAAEI 150
IAHSKPDVFN HNVETVPSLY KTIRPGARYY NSLSLLHSIK KLSSEIFTKS 200
GMMVGLGEKI SEVIQVMDDL REAKVDFLTI GQYLQPTKDH AEVAKYVTPE 250
EFKYLERVAR TKGFLMVSAS PLTRSSYHAD EDFQKLKQNY RQSLVS 296
Length:296
Mass (Da):33,516
Last modified:November 13, 2007 - v1
Checksum:iD7844722EE460D80
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000409 Genomic DNA. Translation: ABV73875.1.
RefSeqiYP_001492660.1. NC_009879.1.

Genome annotation databases

EnsemblBacteriaiABV73875; ABV73875; A1E_04770.
GeneIDi5626573.
KEGGircm:A1E_04770.
PATRICi17886667. VBIRicCan89738_1035.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000409 Genomic DNA. Translation: ABV73875.1 .
RefSeqi YP_001492660.1. NC_009879.1.

3D structure databases

ProteinModelPortali A8EZU2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 293613.A1E_04770.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV73875 ; ABV73875 ; A1E_04770 .
GeneIDi 5626573.
KEGGi rcm:A1E_04770.
PATRICi 17886667. VBIRicCan89738_1035.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi FERIATN.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci RCAN293613:GHI3-950-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Rickettsia canadensis."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: McKiel.

Entry informationi

Entry nameiLIPA_RICCK
AccessioniPrimary (citable) accession number: A8EZU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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