fumC

Functionⁱ

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityⁱ

(S)-malate = fumarate + H₂O.UniRule annotation

Pathwayⁱ: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:

Fumarate hydratase class II (fumC)

This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	187	SubstrateUniRule annotation	1
Active siteⁱ	188	Proton donor/acceptorUniRule annotation	1
Active siteⁱ	318	UniRule annotation	1
Binding siteⁱ	319	SubstrateUniRule annotation	1
Siteⁱ	331	Important for catalytic activityUniRule annotation	1

GO - Molecular functionⁱ

fumarate hydratase activity Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

fumarate metabolic process Source: InterPro
tricarboxylic acid cycle Source: UniProtKB-UniRule

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	LyaseUniRule annotationSAAS annotationImported
Biological process	Tricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

BioCycⁱ	RCAN293613:G1G6U-889-MONOMER.
UniPathwayⁱ	UPA00223; UER01007.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation) Short name: Fumarase CUniRule annotation Alternative name(s): Aerobic fumaraseUniRule annotation Iron-independent fumaraseUniRule annotation
Gene namesⁱ	Name:fumCUniRule annotationImported Ordered Locus Names:A1E_04395Imported
Organismⁱ	Rickettsia canadensis (strain McKiel)Imported
Taxonomic identifierⁱ	293613 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia canadensis
Proteomesⁱ	UP000007056 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_00743

GO - Cellular componentⁱ

tricarboxylic acid cycle enzyme complex Source: InterPro

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

CytoplasmUniRule annotation

Interactionⁱ

Subunit structureⁱ

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	293613.A1E_04395.

STRINGⁱ

293613.A1E_04395.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	A8EZM1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	12 – 342	Lyase_1InterPro annotationAdd BLAST		331
Domainⁱ	408 – 461	FumaraseC_CInterPro annotationAdd BLAST		54

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	98 – 100	Substrate bindingUniRule annotation	3
Regionⁱ	129 – 132	Substrate binding (B site)UniRule annotation	4
Regionⁱ	139 – 141	Substrate bindingUniRule annotation	3
Regionⁱ	324 – 326	Substrate bindingUniRule annotation	3

Sequence similaritiesⁱ

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C9Q. Bacteria. COG0114. LUCA.
HOGENOMⁱ	HOG000061736.
KEGG Orthology (KO) More...KOⁱ	K01679.
OMAⁱ	VSFTDNC.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01XG.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01362. Fumarase_classII. 1 hit.
Gene3Dⁱ	1.10.275.10. 1 hit.
HAMAPⁱ	MF_00743. FumaraseC. 1 hit.
InterProⁱ	View protein in InterPro IPR005677. Fum_hydII. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR020557. Fumarate_lyase_CS. IPR000362. Fumarate_lyase_fam. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like.
PANTHERⁱ	PTHR11444. PTHR11444. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit.
PRINTSⁱ	PR00149. FUMRATELYASE.
SUPFAMⁱ	SSF48557. SSF48557. 1 hit.
TIGRFAMsⁱ	TIGR00979. fumC_II. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00163. FUMARATE_LYASES. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

A8EZM1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKNYRTESDS FGEIQIEEKY YWGAQTQRSL ENFKIGRQKM PEILIRALTI 
        60         70         80         90        100
LKKCAAQVNH EFGDLEAKIA TSIDKATTRI LSGEFNDNFP LGVWQTGSGT 
       110        120        130        140        150
QTNMNMNEVI ASIANEELTG KKGGKSPVHP NDHVNKGQSS NDSFPTGMNI 
       160        170        180        190        200
ATVLATKQQL IPALNNLLTS LQDKSKDWDK IIKIGRTHLQ DATPLTLQQE 
       210        220        230        240        250
FSGYITQIEY ALERIEDALK KVYLLAQGGT AVGTGINSRI GFDIKFAEKV 
       260        270        280        290        300
AEFTKQPFKA APNKFESLAA HDALVEFSGT LNTIAVSLMK IANDIRLLGS 
       310        320        330        340        350
GPRCGLGELH LPENEPGSSI MPGKVNPTQV EALTMVCSQV MGNHVTVTIA 
       360        370        380        390        400
GSNGQLELNV FKPVIIYNIL QSIELLSDSI NSFVTHCIKG LEPNIARIND 
       410        420        430        440        450
LRNQSLMLVT ALNPHIGYDN AAKIAKEAHK HGITLKEAAK KLNFLSEEEF 
       460 
DKIVVPEKMV GQY

Length:463

Mass (Da):50,907

Last modified:November 13, 2007 - v1

Checksum:ⁱA95B1DC0BFE8D779

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000409 Genomic DNA. Translation: ABV73804.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012148999.1. NC_009879.1.

Genome annotation databases

EnsemblBacteriaⁱ	ABV73804; ABV73804; A1E_04395.
KEGGⁱ	rcm:A1E_04395.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A8EZM1	Q9ZCQ4 D5AXR8 UPI0001AF80DC B7PXR2 A0A0C2QYQ7 UPI0002D4B3FD UPI00050964B5 UPI0006A77215 UPI0007DB06A2 UPI000CDE97EF +52	Rickettsia prowazekii (strain Madrid E) Rickettsia prowazekii (strain Rp22) Ixodes scapularis (Black-legged tick) (Deer tick) Rickettsia asembonensis Rickettsia conorii Rickettsia aeschlimannii Rickettsia amblyommatis (Rickettsia amblyommii) Rickettsia sp. Tenjiku01 Rickettsia sp. AUS118 Rickettsia endosymbiont of Ixodes scapularis And more	461	UniRef90_Q9ZCQ4	Cluster: Fumarate hydratase class II	63

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A8EZM1	Q9ZCQ4 D5AXR8 UPI0001AF80DC B7PXR2 UPI00050A0489 UPI000252F68F UPI0007DB06A2 UPI000CDE97EF A0A0F3QFV3 C4K2I5 +671	Rickettsia prowazekii (strain Madrid E) Rickettsia prowazekii (strain Rp22) Ixodes scapularis (Black-legged tick) (Deer tick) Rickettsia tamurae Rickettsia rhipicephali Rickettsia sp. Tenjiku01 Rickettsia sp. AUS118 Rickettsia bellii str. RML Mogi Rickettsia peacockii (strain Rustic) Rickettsia rickettsii (strain Iowa) And more	461	UniRef50_Q9ZCQ4	Cluster: Fumarate hydratase class II	682

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000409 Genomic DNA. Translation: ABV73804.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012148999.1. NC_009879.1.

3D structure databases

ProteinModelPortalⁱ	A8EZM1.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	293613.A1E_04395.

STRINGⁱ

293613.A1E_04395.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ABV73804; ABV73804; A1E_04395.
KEGGⁱ	rcm:A1E_04395.

Phylogenomic databases

eggNOGⁱ	ENOG4105C9Q. Bacteria. COG0114. LUCA.
HOGENOMⁱ	HOG000061736.
KEGG Orthology (KO) More...KOⁱ	K01679.
OMAⁱ	VSFTDNC.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01XG.

Enzyme and pathway databases

UniPathwayⁱ	UPA00223; UER01007.
BioCycⁱ	RCAN293613:G1G6U-889-MONOMER.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01362. Fumarase_classII. 1 hit.
Gene3Dⁱ	1.10.275.10. 1 hit.
HAMAPⁱ	MF_00743. FumaraseC. 1 hit.
InterProⁱ	View protein in InterPro IPR005677. Fum_hydII. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR020557. Fumarate_lyase_CS. IPR000362. Fumarate_lyase_fam. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like.
PANTHERⁱ	PTHR11444. PTHR11444. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit.
PRINTSⁱ	PR00149. FUMRATELYASE.
SUPFAMⁱ	SSF48557. SSF48557. 1 hit.
TIGRFAMsⁱ	TIGR00979. fumC_II. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00163. FUMARATE_LYASES. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	A8EZM1_RICCK
Accessionⁱ	A8EZM1Primary (citable) accession number: A8EZM1
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	November 13, 2007
	Last sequence update:	November 13, 2007
	Last modified:	February 28, 2018
	This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteomeImported

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A8EZM1 (A8EZM1_RICCK)

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Fumarate hydratase class II

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: tricarboxylic acid cycle

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: tricarboxylic acid cycle

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ