UniProtKB - A8EZM1 (A8EZM1_RICCK)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
Fumarate hydratase class II
Gene
fumC
Organism
Rickettsia canadensis (strain McKiel)
Status
Functioni
Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation
Miscellaneous
There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation
Catalytic activityi
(S)-malate = fumarate + H2O.UniRule annotation
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Fumarate hydratase class II (fumC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 187 | SubstrateUniRule annotation | 1 | |
Active sitei | 188 | Proton donor/acceptorUniRule annotation | 1 | |
Active sitei | 318 | UniRule annotation | 1 | |
Binding sitei | 319 | SubstrateUniRule annotation | 1 | |
Sitei | 331 | Important for catalytic activityUniRule annotation | 1 |
GO - Molecular functioni
- fumarate hydratase activity Source: UniProtKB-UniRule
GO - Biological processi
- fumarate metabolic process Source: InterPro
- tricarboxylic acid cycle Source: UniProtKB-UniRule
Keywordsi
Molecular function | LyaseUniRule annotationSAAS annotationImported |
Biological process | Tricarboxylic acid cycleUniRule annotation |
Enzyme and pathway databases
BioCyci | RCAN293613:G1G6U-889-MONOMER. |
UniPathwayi | UPA00223; UER01007. |
Names & Taxonomyi
Protein namesi | Recommended name: Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)Short name: Fumarase CUniRule annotation Alternative name(s): Aerobic fumaraseUniRule annotation Iron-independent fumaraseUniRule annotation |
Gene namesi | Name:fumCUniRule annotationImported Ordered Locus Names:A1E_04395Imported |
Organismi | Rickettsia canadensis (strain McKiel)Imported |
Taxonomic identifieri | 293613 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- tricarboxylic acid cycle enzyme complex Source: InterPro
Keywords - Cellular componenti
CytoplasmUniRule annotationInteractioni
Subunit structurei
Homotetramer.UniRule annotation
Protein-protein interaction databases
STRINGi | 293613.A1E_04395. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 342 | Lyase_1InterPro annotationAdd BLAST | 331 | |
Domaini | 408 – 461 | FumaraseC_CInterPro annotationAdd BLAST | 54 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 98 – 100 | Substrate bindingUniRule annotation | 3 | |
Regioni | 129 – 132 | Substrate binding (B site)UniRule annotation | 4 | |
Regioni | 139 – 141 | Substrate bindingUniRule annotation | 3 | |
Regioni | 324 – 326 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105C9Q. Bacteria. COG0114. LUCA. |
HOGENOMi | HOG000061736. |
KOi | K01679. |
OMAi | VSFTDNC. |
OrthoDBi | POG091H01XG. |
Family and domain databases
CDDi | cd01362. Fumarase_classII. 1 hit. |
Gene3Di | 1.10.275.10. 1 hit. |
HAMAPi | MF_00743. FumaraseC. 1 hit. |
InterProi | View protein in InterPro IPR005677. Fum_hydII. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR020557. Fumarate_lyase_CS. IPR000362. Fumarate_lyase_fam. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. |
PANTHERi | PTHR11444. PTHR11444. 1 hit. |
Pfami | View protein in Pfam PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. |
PRINTSi | PR00149. FUMRATELYASE. |
SUPFAMi | SSF48557. SSF48557. 1 hit. |
TIGRFAMsi | TIGR00979. fumC_II. 1 hit. |
PROSITEi | View protein in PROSITE PS00163. FUMARATE_LYASES. 1 hit. |
i Sequence
Sequence statusi: Complete.
A8EZM1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNYRTESDS FGEIQIEEKY YWGAQTQRSL ENFKIGRQKM PEILIRALTI
60 70 80 90 100
LKKCAAQVNH EFGDLEAKIA TSIDKATTRI LSGEFNDNFP LGVWQTGSGT
110 120 130 140 150
QTNMNMNEVI ASIANEELTG KKGGKSPVHP NDHVNKGQSS NDSFPTGMNI
160 170 180 190 200
ATVLATKQQL IPALNNLLTS LQDKSKDWDK IIKIGRTHLQ DATPLTLQQE
210 220 230 240 250
FSGYITQIEY ALERIEDALK KVYLLAQGGT AVGTGINSRI GFDIKFAEKV
260 270 280 290 300
AEFTKQPFKA APNKFESLAA HDALVEFSGT LNTIAVSLMK IANDIRLLGS
310 320 330 340 350
GPRCGLGELH LPENEPGSSI MPGKVNPTQV EALTMVCSQV MGNHVTVTIA
360 370 380 390 400
GSNGQLELNV FKPVIIYNIL QSIELLSDSI NSFVTHCIKG LEPNIARIND
410 420 430 440 450
LRNQSLMLVT ALNPHIGYDN AAKIAKEAHK HGITLKEAAK KLNFLSEEEF
460
DKIVVPEKMV GQY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000409 Genomic DNA. Translation: ABV73804.1. |
RefSeqi | WP_012148999.1. NC_009879.1. |
Genome annotation databases
EnsemblBacteriai | ABV73804; ABV73804; A1E_04395. |
KEGGi | rcm:A1E_04395. |
Similar proteinsi
Entry informationi
Entry namei | A8EZM1_RICCK | |
Accessioni | A8EZM1Primary (citable) accession number: A8EZM1 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | November 13, 2007 |
Last sequence update: | November 13, 2007 | |
Last modified: | February 28, 2018 | |
This is version 71 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |