ID   LPXB_RICCK              Reviewed;         385 AA.
AC   A8EZC6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   27-MAR-2024, entry version 74.
DE   RecName: Full=Lipid-A-disaccharide synthase {ECO:0000255|HAMAP-Rule:MF_00392};
DE            EC=2.4.1.182 {ECO:0000255|HAMAP-Rule:MF_00392};
GN   Name=lpxB {ECO:0000255|HAMAP-Rule:MF_00392};
GN   OrderedLocusNames=A1E_03910;
OS   Rickettsia canadensis (strain McKiel).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; belli group.
OX   NCBI_TaxID=293613;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=McKiel;
RA   Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Dasch G., Eremeeva M.;
RT   "Complete genome sequence of Rickettsia canadensis.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC       diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor
CC       of lipid A, a phosphorylated glycolipid that anchors the
CC       lipopolysaccharide to the outer membrane of the cell.
CC       {ECO:0000255|HAMAP-Rule:MF_00392}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-
CC         glucosamine = a lipid A disaccharide + H(+) + UDP;
CC         Xref=Rhea:RHEA:67828, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:137748, ChEBI:CHEBI:176338, ChEBI:CHEBI:176343;
CC         EC=2.4.1.182; Evidence={ECO:0000255|HAMAP-Rule:MF_00392};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00392}.
CC   -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00392}.
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DR   EMBL; CP000409; ABV73709.1; -; Genomic_DNA.
DR   RefSeq; WP_012148904.1; NC_009879.1.
DR   AlphaFoldDB; A8EZC6; -.
DR   SMR; A8EZC6; -.
DR   STRING; 293613.A1E_03910; -.
DR   CAZy; GT19; Glycosyltransferase Family 19.
DR   KEGG; rcm:A1E_03910; -.
DR   eggNOG; COG0763; Bacteria.
DR   HOGENOM; CLU_036577_2_0_5; -.
DR   UniPathway; UPA00973; -.
DR   Proteomes; UP000007056; Chromosome.
DR   GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00392; LpxB; 1.
DR   InterPro; IPR003835; Glyco_trans_19.
DR   NCBIfam; TIGR00215; lpxB; 1.
DR   PANTHER; PTHR30372; LIPID-A-DISACCHARIDE SYNTHASE; 1.
DR   PANTHER; PTHR30372:SF4; LIPID-A-DISACCHARIDE SYNTHASE, MITOCHONDRIAL-RELATED; 1.
DR   Pfam; PF02684; LpxB; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipid A biosynthesis; Lipid biosynthesis;
KW   Lipid metabolism; Transferase.
FT   CHAIN           1..385
FT                   /note="Lipid-A-disaccharide synthase"
FT                   /id="PRO_1000049413"
SQ   SEQUENCE   385 AA;  43694 MW;  56A009925EE8D371 CRC64;
     MAKIYFIAGE MSGDFIGGRI IQHLKNNTGV QFVGVGSKYM EEAGSFKSLF PISAINLIGF
     VEILPHILKL KKLIDKTVED IINSKADLLI TIDSPGFTYR VAKQVRKLLP KLKMIHIVAP
     SVWAYKEGRA IKYAKIYDCL FAVLPFESPY FTKVGLDCRY IGHPIMEQEF YSDKIALRKE
     FKIDENEKVL CVTLGSRRGE ILRHLPVFIA SIEEIFESCK NLKVIFTLVN PANEVIIKPF
     LENVKFNYLF SSERLKTYAL SDLALAKSGT NTLEIAASGT PMIVAYKVNI LSFLIIRALI
     KIKYVTLINI IAGSEIIPEF IQHNCRATLI SNKLQELLFS SKKAYKQVIE SQKILQKLRF
     KSNQLPSYIA AEIIKQEFLE PKIKL
//