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A8EZ08

- DAPF_RICCK

UniProt

A8EZ08 - DAPF_RICCK

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Rickettsia canadensis (strain McKiel)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151SubstrateUniRule annotation
    Binding sitei49 – 491SubstrateUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Active sitei75 – 751Proton donor/acceptorUniRule annotation
    Binding sitei155 – 1551SubstrateUniRule annotation
    Sitei157 – 1571Important for catalytic activityUniRule annotation
    Binding sitei187 – 1871SubstrateUniRule annotation
    Sitei204 – 2041Important for catalytic activityUniRule annotation
    Active sitei213 – 2131Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciRCAN293613:GHI3-659-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:A1E_03280
    OrganismiRickettsia canadensis (strain McKiel)
    Taxonomic identifieri293613 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
    ProteomesiUP000007056: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Diaminopimelate epimerasePRO_1000011954Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 213

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi293613.A1E_03280.

    Structurei

    3D structure databases

    ProteinModelPortaliA8EZ08.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni12 – 132Substrate bindingUniRule annotation
    Regioni75 – 773Substrate bindingUniRule annotation
    Regioni204 – 2052Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiRISEMEI.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8EZ08-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISKINFVKM HGLGNDCVIV NKRDLLNAHN LSQLAKNIAD RHTGIGCDQF    50
    IIYENHNNFY EMIIYNIDGS SAKLCGNATR CLAKLIYLDT GKKDITIVVG 100
    NKELLCRIED ENKISVNVGA VSFNETWMPS RDKIWAFAAR YMIDLKETIC 150
    VDVGNPHLVI FSKLESQDQK IVGETLQAKE LFANGVNVNF AEIKDNKINL 200
    SVWERGVGLT GACGSGACGS FAAGLKRGFI HSPSTVVFKH GSLNMKEENG 250
    NIIMQGSATL IAEGVYCCE 269
    Length:269
    Mass (Da):29,584
    Last modified:November 13, 2007 - v1
    Checksum:i68A1E6E4A062CE9F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000409 Genomic DNA. Translation: ABV73591.1.
    RefSeqiYP_001492376.1. NC_009879.1.

    Genome annotation databases

    EnsemblBacteriaiABV73591; ABV73591; A1E_03280.
    GeneIDi5626787.
    KEGGircm:A1E_03280.
    PATRICi17885985. VBIRicCan89738_0702.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000409 Genomic DNA. Translation: ABV73591.1 .
    RefSeqi YP_001492376.1. NC_009879.1.

    3D structure databases

    ProteinModelPortali A8EZ08.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 293613.A1E_03280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV73591 ; ABV73591 ; A1E_03280 .
    GeneIDi 5626787.
    KEGGi rcm:A1E_03280.
    PATRICi 17885985. VBIRicCan89738_0702.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi RISEMEI.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci RCAN293613:GHI3-659-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Rickettsia canadensis."
      Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: McKiel.

    Entry informationi

    Entry nameiDAPF_RICCK
    AccessioniPrimary (citable) accession number: A8EZ08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3