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A8EZ02 (SYL_RICCK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:A1E_03250
OrganismRickettsia canadensis (strain McKiel) [Complete proteome] [HAMAP]
Taxonomic identifier293613 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 834834Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009417

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif602 – 6065"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8EZ02 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: F03D4890B99F6947

FASTA83496,715
        10         20         30         40         50         60 
MNQIEQKWQQ IWDDEKAFEV SNECNKPKYY VLEMLPYPSG KIHVGHVRNY SIGDVIARFM 

        70         80         90        100        110        120 
NMQGFNVLHP MGWDAFGLPA ENAAIKNNVH PKEWTYSNVD NMQQQLKSMG FAYDWSRVIN 

       130        140        150        160        170        180 
SCDPQYYKYE QKFFLELYER NLAYQKEALV NWDPVDNTVL ANEQVVDGRG WRSGAIIEKR 

       190        200        210        220        230        240 
YLKQWFLKIT DYAEELLNEI KNLKEWPEAV RSMQEKWIGK SIGANFYFKV KDNEDAIIEV 

       250        260        270        280        290        300 
FSTKPETIFG ASFIGIAFNH PIIEKLISKT PEISNFITKC LHITGSSELE KAEKDGILTS 

       310        320        330        340        350        360 
LYVIHPFDPS IILPVIITNF VLMDYGTGAI FGCPAHDERD HELAVKMNLP IKQVIEADID 

       370        380        390        400        410        420 
VHKIAYTEDG ILINSDFLNG LTNNEAKQKV IKEIEKLQIG KRSINYRLKD WGISRQRFWG 

       430        440        450        460        470        480 
CPIPMIYCKV CDIVPVPYKD LPVTLPDNVK FNGHGNPLDH HPTWKHVNCP KCDKPAIRET 

       490        500        510        520        530        540 
DTFDTFFESS WYFTRYCNSH ATDMTDKKAC DYWLPVDKYI GGIEHAVMHL LYARFFTKVM 

       550        560        570        580        590        600 
NEQNYVSVRE PFKGLFTQGM VLHATYKDEH NNWLYPDEVV KKDNKFFHKK SGNLVVQGRI 

       610        620        630        640        650        660 
EKMSKSKKNL IDLETMQKQY GADAIRLFVL SDSPPEKDLE WSVSGLEGCS RFINKLEHMF 

       670        680        690        700        710        720 
KVIASLKDDV TGINKELNRL VHLTIKYVAE DIKHFALNRA IARMRELSNA ISSEFSKEVM 

       730        740        750        760        770        780 
DVKTVKYGFN VLVQLLNPFI PHITEEIWQK LGNKERLYKT AFPAFDESML ELDTYVMAVQ 

       790        800        810        820        830 
VNGKLRDTYE FNNSASDYEI KQITINLPKV QKFLEGTEPK KIIFVPRKIV NIIV 

« Hide

References

[1]"Complete genome sequence of Rickettsia canadensis."
Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: McKiel.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000409 Genomic DNA. Translation: ABV73585.1.
RefSeqYP_001492370.1. NC_009879.1.

3D structure databases

ProteinModelPortalA8EZ02.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING293613.A1E_03250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV73585; ABV73585; A1E_03250.
GeneID5626196.
KEGGrcm:A1E_03250.
PATRIC17885973. VBIRicCan89738_0696.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycRCAN293613:GHI3-653-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RICCK
AccessionPrimary (citable) accession number: A8EZ02
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries