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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Rickettsia canadensis (strain McKiel)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871MagnesiumUniRule annotation
Metal bindingi493 – 4931MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciRCAN293613:GHI3-592-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:A1E_02925
OrganismiRickettsia canadensis (strain McKiel)
Taxonomic identifieri293613 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000007056 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 747747Polyribonucleotide nucleotidyltransferasePRO_0000329817Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi293613.A1E_02925.

Structurei

3D structure databases

ProteinModelPortaliA8EYU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KHUniRule annotationAdd
BLAST
Domaini623 – 69169S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8EYU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNEITKSVT WNGKVLELST GKIARQADGS VTVKMGNSVL LCTAVVANTA
60 70 80 90 100
KEGIGFFPLT INYREMAYAV GKIPGGFFKR EGKASEREVL VSRLIDRPIR
110 120 130 140 150
PLFHPAFVNE THVTCTVLSY DPETPVDILA IIGASAALAL SPAPYLEIVA
160 170 180 190 200
ASKVGLINGE FVLNPTLELL KTSQLDLVVA GTTESVMMVE SEAHLLSEAQ
210 220 230 240 250
MLEAVKFGFE SFQPVVKIIK ELAEEAKRPK LEMQDLYPSV LKNEIEQLFA
260 270 280 290 300
KEIEQAFAIK SKQERSTSLD LISEKVITHF ISNIENKKYN NYQIESALKS
310 320 330 340 350
VESDILRKEI LEKNKRIDGR STMDIRQIAC EVGLLPSAHG SALFTRGETQ
360 370 380 390 400
SLVSSTLGTS LDEQIVDNLE GEYKERFMLN YIFPPYSVNE AMPMKAPSRR
410 420 430 440 450
EVGHGKLAWR AINPILPNKV QFPYSIRVVA ETTESNGSSS MATVCGSSLA
460 470 480 490 500
LMQTGIPIKA PVAGIAMGLV KEGKKFAVLS DILGDEDYFG DMDFKVAGTS
510 520 530 540 550
EGITALQMDI KISGVDFKIM KVALEQARLG RLHILEQMNK VISKPNSELS
560 570 580 590 600
KNAPSTTTIK IDKDKIRDII GPGGKIIKEI CETSGAKIDI SDDGTVSVYA
610 620 630 640 650
ADRDKLKIAS DKIKAIAIEP EIGEIFNGTV TKILDSGAFI NYLGNKDGFV
660 670 680 690 700
HISEISEERI DTVSSVIKQG DIVKVKLIGF DNKGKAKLTI KNADKDKSLN
710 720 730 740
NPKPQNSINN AKENSEHVRC DSIKKRAWNE DNNAETAEFI TERKYFN
Length:747
Mass (Da):82,109
Last modified:November 13, 2007 - v1
Checksum:i11AA65929395FD43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000409 Genomic DNA. Translation: ABV73525.1.
RefSeqiWP_012148722.1. NC_009879.1.
YP_001492310.1. NC_009879.1.

Genome annotation databases

EnsemblBacteriaiABV73525; ABV73525; A1E_02925.
KEGGircm:A1E_02925.
PATRICi17885841. VBIRicCan89738_0631.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000409 Genomic DNA. Translation: ABV73525.1.
RefSeqiWP_012148722.1. NC_009879.1.
YP_001492310.1. NC_009879.1.

3D structure databases

ProteinModelPortaliA8EYU2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi293613.A1E_02925.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV73525; ABV73525; A1E_02925.
KEGGircm:A1E_02925.
PATRICi17885841. VBIRicCan89738_0631.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciRCAN293613:GHI3-592-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Rickettsia canadensis."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: McKiel.

Entry informationi

Entry nameiPNP_RICCK
AccessioniPrimary (citable) accession number: A8EYU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 13, 2007
Last modified: May 27, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.