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A8EY49

- SYI_RICCK

UniProt

A8EY49 - SYI_RICCK

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia canadensis (strain McKiel)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei614 – 6141ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciRCAN293613:GHI3-339-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:A1E_01685
    OrganismiRickettsia canadensis (strain McKiel)
    Taxonomic identifieri293613 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
    ProteomesiUP000007056: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10791079Isoleucine--tRNA ligasePRO_1000022160Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi293613.A1E_01685.

    Structurei

    3D structure databases

    ProteinModelPortaliA8EY49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi53 – 6311"HIGH" regionAdd
    BLAST
    Motifi611 – 6155"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiMNIRDAN.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8EY49-1 [UniParc]FASTAAdd to Basket

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    MTNTKYYPEV SSNADFAAIE REILKLWQDN NIFQKSIDNR IKDAEFIFYD     50
    GPPFANGLPH YGHLLTGFIK DVYARYQTIK GKKVERRFGW DCHGLPAEMQ 100
    SEQELGISGR LAITNFSIEK FNSHCRASVM KYTGEWEQYV TRQARWVDFK 150
    NSYKTMDTHF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSHFETR 200
    LDNSYRERAD KAVTVSFMLR DKLPHSEYKE YRIFAWTTTP WTLPANLALA 250
    VGSDIDYALV PKNDICYIIA AASVSKYAKE LELKGDEQFT IIKGSELEGL 300
    RYKPLFNYFE NHPNSFKIFA CDFVVEGDGT GVVHMAPGFG EDDQILCESK 350
    GIELVCPVDN SGKFTKEIPD LEGLQVFDAN DKIIIKLKEQ GNWLKTEQYI 400
    HNYPHCWRTD TPLIYKAVPS WYVKVTNFKD RIVELNQQIN WIPSHVKDNV 450
    FGKWLENARD WSISRNRFWG TPLPVWKSDD PKYPRIDVYG SIEELEKDFG 500
    VKVTDLHRPF IDELTRANPD DPTGKSTMRR IEDVFDCWFE SGSMPYSQAH 550
    YPFENKKWFE DHFPADFIVE YVAQTRGWFY TLMVLSTALF DRPPFLNCIC 600
    HGVILDTTGQ KLSKRLNNYA DPLELFDKYG SDALRITMLS SNVVKGQELL 650
    IDKDGKMVFD TLRLFIKPIW NAYHFFTMYA NADSLKGKSD FASENVLDIY 700
    ILSKLKIAVQ KIEKSLDNFD TQAAYHQVSE FFEVLNNWYI RRSRARFWKS 750
    EKDADKQNAY NTLYSCLETM AIAMSALVPM ISEAIYLGLH NTVIPQLDYG 800
    ISGDKPGAQD PIIKSQDGIR GCRNDNLSVH LCNYPKLSNF EVNHELVATM 850
    DTVLDICSNS LFIRSNENVR VRQPLASITI ISKHNDKLKD FEDLIKDEIN 900
    VKAIIYRDDL ENYATTKLSL NFPMLGKRLP YKMKEIIAAS KKGEWEATAS 950
    ALAICGETLK SEEYKLVLEP YSHIKGAASF ADNSSLLILD LELTPELIKE 1000
    GIARDIVRFI QQARKDADCS ITDRILIEII SESDLSKIIN IYGDYIKEQT 1050
    LSEFAKDFTP DYVNEIELEN HKIQLKIKR 1079
    Length:1,079
    Mass (Da):124,622
    Last modified:November 13, 2007 - v1
    Checksum:i10C7C57F24B79612
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000409 Genomic DNA. Translation: ABV73282.1.
    RefSeqiWP_012148481.1. NC_009879.1.
    YP_001492067.1. NC_009879.1.

    Genome annotation databases

    EnsemblBacteriaiABV73282; ABV73282; A1E_01685.
    GeneIDi5626461.
    KEGGircm:A1E_01685.
    PATRICi17885291. VBIRicCan89738_0367.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000409 Genomic DNA. Translation: ABV73282.1 .
    RefSeqi WP_012148481.1. NC_009879.1.
    YP_001492067.1. NC_009879.1.

    3D structure databases

    ProteinModelPortali A8EY49.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 293613.A1E_01685.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV73282 ; ABV73282 ; A1E_01685 .
    GeneIDi 5626461.
    KEGGi rcm:A1E_01685.
    PATRICi 17885291. VBIRicCan89738_0367.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi MNIRDAN.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci RCAN293613:GHI3-339-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Rickettsia canadensis."
      Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: McKiel.

    Entry informationi

    Entry nameiSYI_RICCK
    AccessioniPrimary (citable) accession number: A8EY49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3