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A8EY49

- SYI_RICCK

UniProt

A8EY49 - SYI_RICCK

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia canadensis (strain McKiel)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei614 – 6141ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRCAN293613:GHI3-339-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:A1E_01685
OrganismiRickettsia canadensis (strain McKiel)
Taxonomic identifieri293613 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000007056: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10791079Isoleucine--tRNA ligasePRO_1000022160Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi293613.A1E_01685.

Structurei

3D structure databases

ProteinModelPortaliA8EY49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiMNIRDAN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8EY49-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNTKYYPEV SSNADFAAIE REILKLWQDN NIFQKSIDNR IKDAEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYQTIK GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEQELGISGR LAITNFSIEK FNSHCRASVM KYTGEWEQYV TRQARWVDFK
160 170 180 190 200
NSYKTMDTHF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSHFETR
210 220 230 240 250
LDNSYRERAD KAVTVSFMLR DKLPHSEYKE YRIFAWTTTP WTLPANLALA
260 270 280 290 300
VGSDIDYALV PKNDICYIIA AASVSKYAKE LELKGDEQFT IIKGSELEGL
310 320 330 340 350
RYKPLFNYFE NHPNSFKIFA CDFVVEGDGT GVVHMAPGFG EDDQILCESK
360 370 380 390 400
GIELVCPVDN SGKFTKEIPD LEGLQVFDAN DKIIIKLKEQ GNWLKTEQYI
410 420 430 440 450
HNYPHCWRTD TPLIYKAVPS WYVKVTNFKD RIVELNQQIN WIPSHVKDNV
460 470 480 490 500
FGKWLENARD WSISRNRFWG TPLPVWKSDD PKYPRIDVYG SIEELEKDFG
510 520 530 540 550
VKVTDLHRPF IDELTRANPD DPTGKSTMRR IEDVFDCWFE SGSMPYSQAH
560 570 580 590 600
YPFENKKWFE DHFPADFIVE YVAQTRGWFY TLMVLSTALF DRPPFLNCIC
610 620 630 640 650
HGVILDTTGQ KLSKRLNNYA DPLELFDKYG SDALRITMLS SNVVKGQELL
660 670 680 690 700
IDKDGKMVFD TLRLFIKPIW NAYHFFTMYA NADSLKGKSD FASENVLDIY
710 720 730 740 750
ILSKLKIAVQ KIEKSLDNFD TQAAYHQVSE FFEVLNNWYI RRSRARFWKS
760 770 780 790 800
EKDADKQNAY NTLYSCLETM AIAMSALVPM ISEAIYLGLH NTVIPQLDYG
810 820 830 840 850
ISGDKPGAQD PIIKSQDGIR GCRNDNLSVH LCNYPKLSNF EVNHELVATM
860 870 880 890 900
DTVLDICSNS LFIRSNENVR VRQPLASITI ISKHNDKLKD FEDLIKDEIN
910 920 930 940 950
VKAIIYRDDL ENYATTKLSL NFPMLGKRLP YKMKEIIAAS KKGEWEATAS
960 970 980 990 1000
ALAICGETLK SEEYKLVLEP YSHIKGAASF ADNSSLLILD LELTPELIKE
1010 1020 1030 1040 1050
GIARDIVRFI QQARKDADCS ITDRILIEII SESDLSKIIN IYGDYIKEQT
1060 1070
LSEFAKDFTP DYVNEIELEN HKIQLKIKR
Length:1,079
Mass (Da):124,622
Last modified:November 13, 2007 - v1
Checksum:i10C7C57F24B79612
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000409 Genomic DNA. Translation: ABV73282.1.
RefSeqiWP_012148481.1. NC_009879.1.
YP_001492067.1. NC_009879.1.

Genome annotation databases

EnsemblBacteriaiABV73282; ABV73282; A1E_01685.
GeneIDi5626461.
KEGGircm:A1E_01685.
PATRICi17885291. VBIRicCan89738_0367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000409 Genomic DNA. Translation: ABV73282.1 .
RefSeqi WP_012148481.1. NC_009879.1.
YP_001492067.1. NC_009879.1.

3D structure databases

ProteinModelPortali A8EY49.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 293613.A1E_01685.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV73282 ; ABV73282 ; A1E_01685 .
GeneIDi 5626461.
KEGGi rcm:A1E_01685.
PATRICi 17885291. VBIRicCan89738_0367.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi MNIRDAN.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RCAN293613:GHI3-339-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Rickettsia canadensis."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: McKiel.

Entry informationi

Entry nameiSYI_RICCK
AccessioniPrimary (citable) accession number: A8EY49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3