Reviewed, UniProtKB/Swiss-Prot A8EY39 (SYE1_RICCK)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	A1E_01635

Organism

Rickettsia canadensis (strain McKiel) [Complete proteome] [HAMAP]

Taxonomic identifier

293613 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367756
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8EY39-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 345DB7B2127C606C	FASTA	463	52,518
	10 20 30 40 50 60 MTNVITRFAP SPTGFLHIGS ARTALFNYLF ARHHNGKFLL RIEDTDKERS TNEAVEAIFS 70 80 90 100 110 120 GLKWLGLDWD GEVIFQSKRN DLYKETALKL LQAGKAYYCF TSQEEIEKQR QKALENKQYF 130 140 150 160 170 180 IFNSDWRDKD PAAYPTDIKP VIRLKTPREG SITIRDTLQG DVVIENSHID DMVLLRSDGT 190 200 210 220 230 240 ATYMLAVVVD DHDMGITHII RGDDHLTNAA RQIAIYQACG YAVPSMTHIP LIHGADGAKL 250 260 270 280 290 300 SKRHGALGVA AYKDMGYLPE SVCNYLLRLG WSHGDDEIIS MDQAIKWFNL DSLGKSPAKL 310 320 330 340 350 360 DFANMNSLNA HYLRLLDNDS ATSKTVERLR QNYNVSKQEV IYINQAIRSL LVRSETLLDL 370 380 390 400 410 420 VQLAQIYLVD SPIIYKQDAK EIIENCDKDL IKQVIENLNK LKQFDKESVQ NKFKEIATHN 430 440 450 460 GLKLNELMKP VRALITGMTA SPSVFEIAEI LGKENILKRL KII

References

[1]	"Complete genome sequence of Rickettsia canadensis." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000409 Genomic DNA. Translation: ABV73272.1.
RefSeq	YP_001492057.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8EY39.
Genome annotation databases
GeneID	5626862.
GenomeReviews	Gene locus A1E_01635 in contig CP000409_GR.
KEGG	rcm:A1E_01635.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MARYDAN.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_RICCK

Accession

Primary (citable) accession number: A8EY39

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)