A8EXI2 (LEP_RICCK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	A1E_00585

Organism

Rickettsia canadensis (strain McKiel) [Complete proteome] [HAMAP]

Taxonomic identifier

293613 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Signal peptidase I		PRO_0000316274
Regions
	Topological domain	1 – 19	19	Cytoplasmic Potential
	Transmembrane	20 – 40	21	Helical; Potential
	Topological domain	41 – 265	225	Periplasmic Potential
Sites
	Active site	44	1	By similarity
	Active site	107	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8EXI2 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: EBC074D99D4DFC28	FASTA	265	31,038
	10 20 30 40 50 60 MQIDTKTNTN KTTAQEWKSF AFVVCIALLI RILIMEPFTV PTGSMKATIL ENDYIFSTKY 70 80 90 100 110 120 SYGYSNYSLS FFDFIPLFKG RIFACEPERG DIVVFRPPND MSVRYIKRLI GLPGDKIQLI 130 140 150 160 170 180 DDIIYINDKK IERTEVGTYI SEEGRKYLKF KETLPNGRTY FSYKLAPIFG VISDDRYGNT 190 200 210 220 230 240 DVFYVPEGKY FFLGDNRDQS NDSRVNLGFV PFENFIAKAQ FIWFSTKITW WDNDIGVINL 250 260 ILKLKPWIES VRLNRIFRNL YNTDE

References

[1]	"Complete genome sequence of Rickettsia canadensis." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: McKiel.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000409 Genomic DNA. Translation: ABV73065.1.
RefSeq	YP_001491850.1. NC_009879.1.
3D structure databases
ProteinModelPortal	A8EXI2.
ModBase	Search...
Protein-protein interaction databases
STRING	A8EXI2.
Protein family/group databases
MEROPS	S26.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5626394.
GenomeReviews	Gene locus A1E_00585 in contig CP000409_GR.
KEGG	rcm:A1E_00585.
PATRIC	17884799. VBIRicCan89738_0128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0681.
HOGENOM	HBG596607.
OMA	KPWIESV.
ProtClustDB	CLSK870751.
Enzyme and pathway databases
BioCyc	RCAN293613:A1E_00585-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits.
KO	K03100.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICCK

Accession

Primary (citable) accession number: A8EXI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program