Reviewed, UniProtKB/Swiss-Prot A8EXI1 (NUOF_RICCK)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	A1E_00580

Organism

Rickettsia canadensis (strain McKiel) [Complete proteome] [HAMAP]

Taxonomic identifier

293613 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	NADH-quinone oxidoreductase subunit F		PRO_0000316286
Regions
	Nucleotide binding	54 – 63	10	NAD By similarity
	Nucleotide binding	166 – 213	48	FMN By similarity
Sites
	Metal binding	345	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	348	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	351	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	391	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8EXI1-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 2CE2FF0A1BF4299C	FASTA	420	46,061
	10 20 30 40 50 60 MLKEEDKIFI NLHGQQSHDL KSSKKRGDWD NTKALLDKGR DFIIEEVKQS GLRGRGGAGF 70 80 90 100 110 120 STGMKWSFMP KNSEKPCYLV VNADESEPGT CKDRDILRFE PHKLIEGCLL ASFAIGANNC 130 140 150 160 170 180 YIYIRGEFYN EASNIQRALD EAYKDGLIGK NACGSGFDCN IYLHCGAGAY ICGEETALLE 190 200 210 220 230 240 SLEGKKGMPR LKPPFPASFG LYGCPTTINN VESIAVVPTI LRRGANWFAS IGKPNNTGTK 250 260 270 280 290 300 VFCISGHVNK PCNVEEVMGI PLKELIEKYA GGVQGGWNNL KAIIPGGSSV PLIPKSICET 310 320 330 340 350 360 VDMDFDSLRT AGSGLGTGGI IVMNQSTDIV YAIARLSKFY MHESCGQCTP CREGTGWMWR 370 380 390 400 410 420 VMMRLVKGNA KKSEIDQLLS VTKAIEGHTI CALGDAAAWP IQGLIRHFRD EIEERICHLM

References

[1]	"Complete genome sequence of Rickettsia canadensis." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000409 Genomic DNA. Translation: ABV73064.1.
RefSeq	YP_001491849.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5626393.
GenomeReviews	Gene locus A1E_00580 in contig CP000409_GR.
KEGG	rcm:A1E_00580.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8EXI1. AMPVRAM.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_RICCK

Accession

Primary (citable) accession number: A8EXI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)