A8EXH7 (A8EXH7_RICCK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605

Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605

Gene names

Name:	trmD HAMAP MF_00605 EMBL ABV73060.1
Ordered Locus Names:	A1E_00560

Organism

Rickettsia canadensis (strain McKiel) [Complete proteome] [HAMAP]

Taxonomic identifier

293613 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605 RuleBase RU003464
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605 S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. RuleBase RU003464
Subunit structure	Homodimer By similarity. HAMAP MF_00605 RuleBase RU003464
Subcellular location	Cytoplasm By similarity HAMAP MF_00605 RuleBase RU003464.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605 RuleBase RU003464

Ontologies

Keywords
Biological process	tRNA processing HAMAP MF_00605 RuleBase RU003464
Cellular component	Cytoplasm HAMAP MF_00605 RuleBase RU003464
Ligand	S-adenosyl-L-methionine HAMAP MF_00605 RuleBase RU003464
Molecular function	Methyltransferase HAMAP MF_00605 RuleBase RU003464 EMBL ABV73060.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	121 – 126	6	S-adenosyl-L-methionine binding By similarity HAMAP MF_00605
Sites
	Binding site	101	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

	Sequence		Length	Mass (Da)
	A8EXH7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 123B54BCAC004EB4	FASTA	220	24,798
	10 20 30 40 50 60 MFPGTLGYSL AGQALHKNIW SYDIINIRDF GLTKHKNIDD AAYGGGDGLI MRPDVLGNSI 70 80 90 100 110 120 DHALSLNSNA KIYYPSPRGR VFTQSFAKEM IKTKNIIFLC GRYEGIDERV IQEYSIMEIS 130 140 150 160 170 180 VGDYILSGGE IPTLTILDCL IRLLPGVLIN QNTLSSESFE EDGEFKGGLE CGLYTRPEVW 190 200 210 220 RGIKVPEILL SGNHKLINEW KREQSRMITK SRRPELLKDL

References

[1]	"Complete genome sequence of Rickettsia canadensis." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000409 Genomic DNA. Translation: ABV73060.1.
RefSeq	YP_001491845.1. NC_009879.1.
3D structure databases
ProteinModelPortal	A8EXH7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8EXH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5626700.
GenomeReviews	Gene locus A1E_00560 in contig CP000409_GR.
KEGG	rcm:A1E_00560.
PATRIC	17884789. VBIRicCan89738_0123.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	RCAN293613:A1E_00560-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8EXH7_RICCK

Accession

Primary (citable) accession number: A8EXH7

Entry history

Integrated into UniProtKB/TrEMBL:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)