A8EX78 (LPXD_RICCK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	A1E_00040

Organism

Rickettsia canadensis (strain McKiel) [Complete proteome] [HAMAP]

Taxonomic identifier

293613 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000050958
Sites
	Active site	253	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8EX78 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 9E99FB952C71968F	FASTA	342	36,897
	10 20 30 40 50 60 MASSNFYKNL GPRKLTTIID FLHDIIEHPK IYEDIVIHDI KILQEASRND ISFLSNTKYS 70 80 90 100 110 120 EFLKTTNAAA CIVPKNFKGE VNPNTILIHA ENSYFAYGKL IDFFYAPIKS YPAKIMKSAI 130 140 150 160 170 180 VAASAIIGKN CYIGHNVVIE DDVIIGDNSI IEAGSFIGRG VNLGRNARIE QHVSINYAII 190 200 210 220 230 240 GDDVVILTGA KIGQDGFGFS TEKGVHHQIF HTGIVKIGNN VKIGANTTID RGSLQDTIIE 250 260 270 280 290 300 DLCCIDNLVQ IGHGVKIGKG SIIIAQVGIA GSSTIGKYCA LGGQVGIAGH LNIGDQVQVA 310 320 330 340 AQSGVAQNIE AGKIVGGSPA VHIMNWHRQS IIMKQLIKKR PK

References

[1]	"Complete genome sequence of Rickettsia canadensis." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: McKiel.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000409 Genomic DNA. Translation: ABV72961.1.
RefSeq	YP_001491746.1. NC_009879.1.
3D structure databases
ProteinModelPortal	A8EX78.
ModBase	Search...
Protein-protein interaction databases
STRING	A8EX78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5626513.
GenomeReviews	Gene locus A1E_00040 in contig CP000409_GR.
KEGG	rcm:A1E_00040.
PATRIC	17884551. VBIRicCan89738_0008.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	RCAN293613:A1E_00040-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_RICCK

Accession

Primary (citable) accession number: A8EX78

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program