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A8EWX7

- BIOB_ARCB4

UniProt

A8EWX7 - BIOB_ARCB4

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Protein
Biotin synthase
Gene
bioB, Abu_2237
Organism
Arcobacter butzleri (strain RM4018)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi19 – 191Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi23 – 231Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi26 – 261Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi63 – 631Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi156 – 1561Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciABUT367737:GHWO-2235-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Abu_2237
OrganismiArcobacter butzleri (strain RM4018)
Taxonomic identifieri367737 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter
ProteomesiUP000001136: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Biotin synthaseUniRule annotation
PRO_0000381207Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi367737.Abu_2237.

Structurei

3D structure databases

ProteinModelPortaliA8EWX7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A8EWX7-1 [UniParc]FASTAAdd to Basket

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MSDNKIYLCA ISNIESGTCN EDCKFCTQSV KYKADIERYR RKEIEDIVNE    50
AKKARANKAV GFCLVTAGTG LDDKRLDYVC RAADAVHKAV PDISLIACNG 100
IASYEQLKEL KKHGIENYNH NLETAREFYN EICTTHSWDD RYETCLNAKK 150
AGLYLCTGGI FGLGETQENR ISMLKSIASL EPMSVPINFF HPNDALPLVK 200
NPLTKQEAFD LVKLARSYLP NQMLMIAGGR ELMFGEEQYD VFKHGANALV 250
VGDYLTTGGR DAQDDIDAVT ALGYEIAFAC HQ 282
Length:282
Mass (Da):31,448
Last modified:November 13, 2007 - v1
Checksum:iF17B187BCA5718FC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000361 Genomic DNA. Translation: ABV68450.1.
RefSeqiYP_001491120.1. NC_009850.1.

Genome annotation databases

EnsemblBacteriaiABV68450; ABV68450; Abu_2237.
GeneIDi5624741.
KEGGiabu:Abu_2237.
PATRICi20965840. VBIArcBut20197_2181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000361 Genomic DNA. Translation: ABV68450.1 .
RefSeqi YP_001491120.1. NC_009850.1.

3D structure databases

ProteinModelPortali A8EWX7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 367737.Abu_2237.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV68450 ; ABV68450 ; Abu_2237 .
GeneIDi 5624741.
KEGGi abu:Abu_2237.
PATRICi 20965840. VBIArcBut20197_2181.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ABUT367737:GHWO-2235-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM4018.

Entry informationi

Entry nameiBIOB_ARCB4
AccessioniPrimary (citable) accession number: A8EWX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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