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A8EWX7

- BIOB_ARCB4

UniProt

A8EWX7 - BIOB_ARCB4

Protein

Biotin synthase

Gene

bioB

Organism
Arcobacter butzleri (strain RM4018)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi19 – 191Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi23 – 231Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi26 – 261Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi98 – 981Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi156 – 1561Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciABUT367737:GHWO-2235-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Abu_2237
    OrganismiArcobacter butzleri (strain RM4018)
    Taxonomic identifieri367737 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter
    ProteomesiUP000001136: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 282282Biotin synthasePRO_0000381207Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi367737.Abu_2237.

    Structurei

    3D structure databases

    ProteinModelPortaliA8EWX7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiTCENTLR.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8EWX7-1 [UniParc]FASTAAdd to Basket

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    MSDNKIYLCA ISNIESGTCN EDCKFCTQSV KYKADIERYR RKEIEDIVNE    50
    AKKARANKAV GFCLVTAGTG LDDKRLDYVC RAADAVHKAV PDISLIACNG 100
    IASYEQLKEL KKHGIENYNH NLETAREFYN EICTTHSWDD RYETCLNAKK 150
    AGLYLCTGGI FGLGETQENR ISMLKSIASL EPMSVPINFF HPNDALPLVK 200
    NPLTKQEAFD LVKLARSYLP NQMLMIAGGR ELMFGEEQYD VFKHGANALV 250
    VGDYLTTGGR DAQDDIDAVT ALGYEIAFAC HQ 282
    Length:282
    Mass (Da):31,448
    Last modified:November 13, 2007 - v1
    Checksum:iF17B187BCA5718FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000361 Genomic DNA. Translation: ABV68450.1.
    RefSeqiYP_001491120.1. NC_009850.1.

    Genome annotation databases

    EnsemblBacteriaiABV68450; ABV68450; Abu_2237.
    GeneIDi5624741.
    KEGGiabu:Abu_2237.
    PATRICi20965840. VBIArcBut20197_2181.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000361 Genomic DNA. Translation: ABV68450.1 .
    RefSeqi YP_001491120.1. NC_009850.1.

    3D structure databases

    ProteinModelPortali A8EWX7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 367737.Abu_2237.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV68450 ; ABV68450 ; Abu_2237 .
    GeneIDi 5624741.
    KEGGi abu:Abu_2237.
    PATRICi 20965840. VBIArcBut20197_2181.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi TCENTLR.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci ABUT367737:GHWO-2235-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RM4018.

    Entry informationi

    Entry nameiBIOB_ARCB4
    AccessioniPrimary (citable) accession number: A8EWX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3