A8EWN0 (DXS_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Abu_2139

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000059443

Sequences

	Sequence		Length	Mass (Da)
	A8EWN0 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: E5618EDB3C0BE8E1	FASTA	601	67,101
	10 20 30 40 50 60 MDIKDKSLKE LEELSSQIRE RIIDVVSRKG GHFSSTLGAV ELTLGMHYVF DAYSDPFIFD 70 80 90 100 110 120 VSHQCYPHKL LTSRWDEFET IRQFGGLSGF TKPKESPADY FVAGHSSTSI SLAVGAAKSI 130 140 150 160 170 180 KLKKENKVPI VMIGDGSMSA GMVYEALNEL GDLKLPVVII LNDNEMSIAK PIGAISKYLS 190 200 210 220 230 240 KILAGKYYQS FKSKVDKFIR KNMPEGTTYL AKRFEEAFKL ITPGILFEEM GIDYIGPIDG 250 260 270 280 290 300 HDLKEVIETL QIAKAMQKPV IVHAHTIKGK GYKIAEGQHE EWHGVGPFNI EDGEFIKKEA 310 320 330 340 350 360 PKSATAVFSD ALLNLACKYE NVVGVTAAMP SGTGINKLMD EFPERFWDVA IAEQHAITSM 370 380 390 400 410 420 AAMAKEGFKP FITIYSTFLQ RGFDQIIHDV CLMNLPVVFA MDRAGIVGND GETHQGAFDI 430 440 450 460 470 480 SFLRFIPNMV LFAPRDNETL EQSLEFAYNL NNPCAIRYPR GAFKKLNFVP TPFVLGKAEI 490 500 510 520 530 540 LKTGISNKLF IGYGAGVSRA IDTEALHNED ITVVDLRFVK PLDKNLLLEL SKKYNDWYIF 550 560 570 580 590 600 SDSQKQSGVG SAILEFLNDE KIHNVQLTSF EYNDKFIEHG DTKLVEQSLG LLPEQLVQKI K

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM4018.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV68353.1.
RefSeq	YP_001491023.1. NC_009850.1.
3D structure databases
ProteinModelPortal	A8EWN0.
ModBase	Search...
Protein-protein interaction databases
STRING	A8EWN0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5624003.
GenomeReviews	Gene locus Abu_2139 in contig CP000361_GR.
KEGG	abu:Abu_2139.
PATRIC	20965642. VBIArcBut20197_2082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	YPRGAFI.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	ABUT367737:ABU_2139-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ARCB4

Accession

Primary (citable) accession number: A8EWN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program