Reviewed, UniProtKB/Swiss-Prot A8EWI6 (SYA_ARCB4)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Abu_2092

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	851 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 851	851	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347491

Sequences

	Sequence		Length	Mass (Da)
	A8EWI6-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 7005E6118CA73F87	FASTA	851	94,966
	10 20 30 40 50 60 MDIRKEYLEF FRSKGHEVIS SMPLVPDDPT LMFTNAGMVQ FKDIFTGAVP TPKNKRATSC 70 80 90 100 110 120 QLCVRAGGKH NDLENVGYTA RHHTLFEMLG NFSFGDYFKE DAIAYAWEFV TVNLALPIDK 130 140 150 160 170 180 LWVTVHNNDD EAFDIWSKYI NPSRIMRFGD KDNFWSMGDT GACGPCSEIF YDQGEENFNG 190 200 210 220 230 240 EEDYMGGDGD RFLEIWNLVF MQYERTADGK LNPLPKPSID TGMGLERVIA IKEGVFNNFD 250 260 270 280 290 300 SSNFKPIIKK IEEISSKNAT SENIGSYRVI ADHLRACSFM LSQGILFGNE GRPYVLRRIL 310 320 330 340 350 360 RRAVRHGYLI GFRKPFMAKL LDTLIEIMGG HYTELVENKN FIEEQLTLEE DRFFKTIDLG 370 380 390 400 410 420 MSLFNEELEK TKDIFSGVTA FKLYDTYGFP LDLTEDMLRD RGLKVDLAKF DELMNNQKAM 430 440 450 460 470 480 AKAAWKGSGD TSNEGDFKQL LEKFGSNEFV GYNNTTYKSK IIALLDEHFK EVKILEKDST 490 500 510 520 530 540 GWVMLDKTPF YATSGGQNGD IGALEDNKHI AIVEETTKFH GLNLSKVKVV NSSLKQGESV 550 560 570 580 590 600 DAIVVNRNEV AKHHSATHLL QSALKIVLGD TVSQAGSLND ASRLRFDFTY PKAMTKEQID 610 620 630 640 650 660 EVEDLVNSMI ARGISGNVEE LPIEQAKKKG AIAMFGEKYG DVVRVVSFED VSVEFCGGTH 670 680 690 700 710 720 VRNTADIGSF YIVKESGVSA GVRRIEAVCG TAAIKYTKDI ISKMNEIQAE VKNSDVILGI 730 740 750 760 770 780 KKLKEQIKDL KKEIETSQSK TSSPIEETII NDTKVIVSVV ENGDLKKIVD DTKNANEKVA 790 800 810 820 830 840 IFLLQAKDDK VLIVAGSKNT NIKAGDWIKN IAPIVGGGGG GRPDFAQAGG KDTSKIQEAK 850 TKALDYAKEN L

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000361 Genomic DNA. Translation: ABV68309.1.
RefSeq	YP_001490979.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8EWI6.
Genome annotation databases
GeneID	5623611.
GenomeReviews	Gene locus Abu_2092 in contig CP000361_GR.
KEGG	abu:Abu_2092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ARCB4

Accession

Primary (citable) accession number: A8EWI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)