A8EWD7 (IF2_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2

Gene names

Name:	infB
Ordered Locus Names:	Abu_2043

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter › Arcobacter butzleri

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 890	890	Translation initiation factor IF-2 HAMAP-Rule MF_00100		PRO_0000335455
Regions
	Nucleotide binding	396 – 403	8	GTP By similarity
	Nucleotide binding	442 – 446	5	GTP By similarity
	Nucleotide binding	496 – 499	4	GTP By similarity
	Region	390 – 538	149	G-domain HAMAP-Rule MF_00100

Sequences

	Sequence		Length	Mass (Da)
	A8EWD7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 59D97729EA9DF258	FASTA	890	97,262
	10 20 30 40 50 60 MSDTVRVYEI AEEAGASSQD VIAKAKDLGI ELKSPQTAVS YEDAEEITKY MMTGKSERLA 70 80 90 100 110 120 TKPAKVKKVV KKEEVKKETE EIETPKEKIE TVQKVEKEII KKPELKKVEI SKPISKAPQK 130 140 150 160 170 180 SEEESENLEN PNKIVPKRKG LVIIKKKRPK EEELEEQQTI TENQSKKQMK SLSEILGGVD 190 200 210 220 230 240 DEEKSYNEPK NKENDDIKKQ KVKKEKKKPL IKTQDHGKKL DVDREYSDEF ASSDDSLLGE 250 260 270 280 290 300 EIVLLDMDLS DSYKIFDEPK PQNIVNQSRS SKPAAFGNVP QGLKRGKRKK RIVRTQEKAE 310 320 330 340 350 360 ITSVTIPEDI RVYEFAEACG KSPAEVITVL FSLGMMVTKN DFLKQDELEI LGEEFGIEVT 370 380 390 400 410 420 VKDALEDVNY VETYNDEEDI DTSSFVTRPP VVTIMGHVDH GKTSLLDKIR SSKVAAGEAG 430 440 450 460 470 480 GITQHITSYT VTKNGQEITF VDTPGHAAFS AMRARGANVT DIIIIVVAAD DGVKMQTEEV 490 500 510 520 530 540 ISHAKASGCP IIVAMNKMDK ETANPDMVKA QMAEKGLTPI DWGGDIEFIG VSARTGDGIE 550 560 570 580 590 600 DLLENILLQA EILELKADPT AKAKATVIEA SLEKGRGPVA NVIVQNGTLK IGDNIVCDTT 610 620 630 640 650 660 FGRVKAITDD NGKPVKELGL SQTGTVLGLN EVPTTGSVLV AMDTEKEVRE IATTRAEHAR 670 680 690 700 710 720 AKELSKSTKV SLEEMSGLIA EGKIKQLPVI IKADVGGSLE AIKGSLEKIA NDEVKVKVVH 730 740 750 760 770 780 AAVGGITESD LVLAGASGEC IILGFNVRPT GSVKAKAKAD GVTINTYSII YDLIDDVKHA 790 800 810 820 830 840 LSGMMSAVIR EENTGQAEVR DTFVVPKVGT VAGCLVTDGK VIRGGHARII RDGVVTYTGK 850 860 870 880 890 ISSLKRFKDD VKEVANGYEC GIMFDKFNDI KVGDFIETFI QIEEKVSVDD

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM4018.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV68260.1.
RefSeq	YP_001490930.1. NC_009850.1.
3D structure databases
ProteinModelPortal	A8EWD7.
SMR	A8EWD7. Positions 791-883.
ModBase	Search...
Protein-protein interaction databases
STRING	367737.Abu_2043.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV68260; ABV68260; Abu_2043.
GeneID	5625285.
KEGG	abu:Abu_2043.
PATRIC	20965452. VBIArcBut20197_1990.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000076906.
KO	K02519.
OMA	IGAYKVN.
ProtClustDB	CLSK2795158.
Enzyme and pathway databases
BioCyc	ABUT367737:GHWO-2020-MONOMER.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF-2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 2 hits. PF11987. IF-2. 1 hit. PF04760. IF2_N. 2 hits. [Graphical view]
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2_ARCB4

Accession

Primary (citable) accession number: A8EWD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program