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A8EWD7 (IF2_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:Abu_2043
OrganismArcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]
Taxonomic identifier367737 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_0000335455

Regions

Nucleotide binding396 – 4038GTP By similarity
Nucleotide binding442 – 4465GTP By similarity
Nucleotide binding496 – 4994GTP By similarity
Region390 – 538149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
A8EWD7 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 59D97729EA9DF258

FASTA89097,262
        10         20         30         40         50         60 
MSDTVRVYEI AEEAGASSQD VIAKAKDLGI ELKSPQTAVS YEDAEEITKY MMTGKSERLA 

        70         80         90        100        110        120 
TKPAKVKKVV KKEEVKKETE EIETPKEKIE TVQKVEKEII KKPELKKVEI SKPISKAPQK 

       130        140        150        160        170        180 
SEEESENLEN PNKIVPKRKG LVIIKKKRPK EEELEEQQTI TENQSKKQMK SLSEILGGVD 

       190        200        210        220        230        240 
DEEKSYNEPK NKENDDIKKQ KVKKEKKKPL IKTQDHGKKL DVDREYSDEF ASSDDSLLGE 

       250        260        270        280        290        300 
EIVLLDMDLS DSYKIFDEPK PQNIVNQSRS SKPAAFGNVP QGLKRGKRKK RIVRTQEKAE 

       310        320        330        340        350        360 
ITSVTIPEDI RVYEFAEACG KSPAEVITVL FSLGMMVTKN DFLKQDELEI LGEEFGIEVT 

       370        380        390        400        410        420 
VKDALEDVNY VETYNDEEDI DTSSFVTRPP VVTIMGHVDH GKTSLLDKIR SSKVAAGEAG 

       430        440        450        460        470        480 
GITQHITSYT VTKNGQEITF VDTPGHAAFS AMRARGANVT DIIIIVVAAD DGVKMQTEEV 

       490        500        510        520        530        540 
ISHAKASGCP IIVAMNKMDK ETANPDMVKA QMAEKGLTPI DWGGDIEFIG VSARTGDGIE 

       550        560        570        580        590        600 
DLLENILLQA EILELKADPT AKAKATVIEA SLEKGRGPVA NVIVQNGTLK IGDNIVCDTT 

       610        620        630        640        650        660 
FGRVKAITDD NGKPVKELGL SQTGTVLGLN EVPTTGSVLV AMDTEKEVRE IATTRAEHAR 

       670        680        690        700        710        720 
AKELSKSTKV SLEEMSGLIA EGKIKQLPVI IKADVGGSLE AIKGSLEKIA NDEVKVKVVH 

       730        740        750        760        770        780 
AAVGGITESD LVLAGASGEC IILGFNVRPT GSVKAKAKAD GVTINTYSII YDLIDDVKHA 

       790        800        810        820        830        840 
LSGMMSAVIR EENTGQAEVR DTFVVPKVGT VAGCLVTDGK VIRGGHARII RDGVVTYTGK 

       850        860        870        880        890 
ISSLKRFKDD VKEVANGYEC GIMFDKFNDI KVGDFIETFI QIEEKVSVDD 

« Hide

References

[1]"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri."
Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E.
PLoS ONE 2:E1358-E1358(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RM4018.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000361 Genomic DNA. Translation: ABV68260.1.
RefSeqYP_001490930.1. NC_009850.1.

3D structure databases

ProteinModelPortalA8EWD7.
SMRA8EWD7. Positions 791-883.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING367737.Abu_2043.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV68260; ABV68260; Abu_2043.
GeneID5625285.
KEGGabu:Abu_2043.
PATRIC20965452. VBIArcBut20197_1990.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076906.
KOK02519.
OMAIQRPTHI.
OrthoDBEOG67HJSV.
ProtClustDBCLSK2795158.

Enzyme and pathway databases

BioCycABUT367737:GHWO-2041-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 2 hits.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_ARCB4
AccessionPrimary (citable) accession number: A8EWD7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries