Reviewed, UniProtKB/Swiss-Prot A8EW04 (MRAY_ARCB4)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Abu_1899

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000057273
Regions
	Transmembrane	16 – 36	21	Potential
	Transmembrane	64 – 84	21	Potential
	Transmembrane	88 – 108	21	Potential
	Transmembrane	130 – 150	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	198 – 218	21	Potential
	Transmembrane	228 – 248	21	Potential
	Transmembrane	256 – 276	21	Potential
	Transmembrane	281 – 301	21	Potential
	Transmembrane	330 – 350	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A8EW04-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: EC9BA6505EFE4795	FASTA	353	39,462
	10 20 30 40 50 60 MFYWLYRHLE INILQYISVR AGISFFIAFV LTMYLMPKFI RWARAKKASQ PIYELAPENH 70 80 90 100 110 120 KIKAGTPTMG GVVFIFSTII ATVLTAKLNN FYIVGGILTL ALFSLIGIQD DYSKISKEKN 130 140 150 160 170 180 SAGLTPRMKL IFQFLCAASI AGILFLYGHS TELFTPFYKF PLFEMGVFGI VFWMFVIVGS 190 200 210 220 230 240 SNAVNLTDGL DGLATVPSIL AFSTLSILVY VVGHAVFANY LLFPNIQIAG ELAIMGSAIC 250 260 270 280 290 300 GALIAFLWFN SHPAEVFMGD SGSLPLGAFM GYLAIVAKSE ILLLAIGFIF VWETVSVILQ 310 320 330 340 350 VGSYKLRQKR VFLMAPIHHH FEQKGWKENK IIVRFWIIAF MSNLIALLSL KIR

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000361 Genomic DNA. Translation: ABV68127.1.
RefSeq	YP_001490797.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8EW04.
Genome annotation databases
GeneID	5623664.
GenomeReviews	Gene locus Abu_1899 in contig CP000361_GR.
KEGG	abu:Abu_1899.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ARCB4

Accession

Primary (citable) accession number: A8EW04

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)