Reviewed, UniProtKB/Swiss-Prot A8EVP8 (PANB_ARCB4)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase
      Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	Abu_1775

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_1000096940
Regions
	Region	50 – 51	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Metal binding	50	1	Magnesium By similarity
	Metal binding	89	1	Magnesium By similarity
	Metal binding	120	1	Magnesium By similarity
	Binding site	89	1	Alpha-ketoisovalerate By similarity
	Binding site	118	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8EVP8-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: C4E53F57B5C5E7C9	FASTA	269	29,857
	10 20 30 40 50 60 MSIIKNNFEK MNITKIKNSK NNKKLTVITA YDALFAKLFE EIADMILVGD SLNMSFAGRP 70 80 90 100 110 120 DTLSATLEQM IYHTNAVCNG AKNAFVIIDM PFGTYINKDE ALKNCVEVYR QTNANAVKIE 130 140 150 160 170 180 GGEDKADIIK HLTSNAVAVM GHIGLMPQYV RSEGGYKVRG KTKEDEEQLI RDAIAVEKAG 190 200 210 220 230 240 AFSIVVEGVK SDVAKKITQA VNIPIIGIGA GVDTDGQVLV WSDMLGFFEE FKPKFVRHYL 250 260 DGAELVKEAV NQYRNDVQDK SFPSKEEEY

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000361 Genomic DNA. Translation: ABV68021.1.
RefSeq	YP_001490691.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8EVP8.
Genome annotation databases
GeneID	5625318.
GenomeReviews	Gene locus Abu_1775 in contig CP000361_GR.
KEGG	abu:Abu_1775.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YATPEQT.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_ARCB4

Accession

Primary (citable) accession number: A8EVP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)