Reviewed, UniProtKB/Swiss-Prot A8EV24 (KATG_ARCB4)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Abu_1548

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Catalase-peroxidase HAMAP MF_01961		PRO_0000354721
Sites
	Active site	96	1	Proton acceptor By similarity
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Site	92	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	95 ↔ 223		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249) By similarity
	Cross-link	223 ↔ 249		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8EV24-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 5459DB4B5E9D6BC0	FASTA	735	81,448
	10 20 30 40 50 60 MAGKCPMGFG TTNPMVRNGG TSNKDWWPNQ LNLKILSQHS NKVNPLGSDF DYAKEFSKLD 70 80 90 100 110 120 YDALKADLTA LMTDSQDWWP ADYGHYGPLF IRMAWHSAGT YRTGDGRGGA STGSQRLAPL 130 140 150 160 170 180 NSWPDNANLD KARRLLWPIK QKYGNKISWA DLMILAGNVA LESMGLKTFG FSGGRVDVWE 190 200 210 220 230 240 PEEDIYWGKE AQWLATSDKE NSRYSGDRDL ENPLAAVQMG LIYVNPEGPD GVPDPIKSGI 250 260 270 280 290 300 DIRETFARMA MDDEETVALT AGGHTFGKCH GAGDAANVGA EPEAEGLVAQ GLGWLSKFLS 310 320 330 340 350 360 GKGDDTITSG IEGSWTANPT RWDNEYFDIL LSYDWELTKS PAGAWQWIPK NPKEEHLAPA 370 380 390 400 410 420 AHDKTKKVTT IMTTADMAMK MDPIYAKISK RFHENPQEFA DAFARAWFKL THRDLGPKSK 430 440 450 460 470 480 YIGPEIPKED LIWQDPIPPI NYEIIDEKDI EILKEKLLSS SLGVSKLVSL AWASASTYRD 490 500 510 520 530 540 SDKRGGANGA RIALEPQRSW ESNSYLNLDE SLKILETIKG EFNSSNSNKK VSLADLIVLG 550 560 570 580 590 600 GCAAVEKAAK DAGFNIKVPF TAGRADATQE QTHVESFSHL EPIADGFRNY SKAKYTLSTE 610 620 630 640 650 660 ELLIDKAQLL SLTIPEMIVL VGGMRVLGAN YANSDLGVFT SNVGVLSNDF FVNLLDMKTA 670 680 690 700 710 720 WYPTTQEEDS FVGKDRQSGS MKYSASRVDL LFGSNSQLRA VSEVYAQEDS KEKFVQDFIN 730 AWTKVMNLDR FDIKK

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000361 Genomic DNA. Translation: ABV67797.1.
RefSeq	YP_001490467.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8EV24.
Genome annotation databases
GeneID	5623902.
GenomeReviews	Gene locus Abu_1548 in contig CP000361_GR.
KEGG	abu:Abu_1548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KTGEPRW.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ARCB4

Accession

Primary (citable) accession number: A8EV24

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)