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A8ETQ3 (DNLJ_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:Abu_1067
OrganismArcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]
Taxonomic identifier367737 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648DNA ligase HAMAP MF_01588
PRO_0000313115

Regions

Domain573 – 64876BRCT
Nucleotide binding30 – 345NAD By similarity
Nucleotide binding79 – 802NAD By similarity

Sites

Active site1101N6-AMP-lysine intermediate By similarity
Metal binding3981Zinc By similarity
Metal binding4011Zinc By similarity
Metal binding4141Zinc By similarity
Metal binding4191Zinc By similarity
Binding site1311NAD By similarity
Binding site1651NAD By similarity
Binding site2801NAD By similarity
Binding site3041NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A8ETQ3 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 9ADB213B65C4D8AB

FASTA64873,519
        10         20         30         40         50         60 
MTKEEYDLNI EKLISWANAY YVFDNPIATD EEYDKLARLC LAYEQENPKL SHPNSPNKRV 

        70         80         90        100        110        120 
GGIVLDGFIK ASHLSRMWSQ EDVFNTQELE DWIKRASKVN TNLEFFCQPK FDGASLNLIY 

       130        140        150        160        170        180 
ENGILKQAIT RGDGTVGEDV TNNAKTIFSI PLEIEEKSLI EIRGEVVIKK ADFEKINIER 

       190        200        210        220        230        240 
AKNGEQLFAN PRNAAAGSLR QLDSNITAKR KLFFNVWGIG QNSLEHKKYS QIMEYIYSLG 

       250        260        270        280        290        300 
FERPQMQTLA SNIEEIESLY HKFISIRNDF EMMLDGMVIK IDDIETQEEL GYTVKFPRWS 

       310        320        330        340        350        360 
CAYKFPAVEK TTKLKAITLQ VGRTGIITPV AEVEPTLIDG STVSRATLHN FDEIERLDLK 

       370        380        390        400        410        420 
INDEVIIIKS GDIIPKITKV FFERRKGDEI TISRPTSCPT CNSEVLDEGT MIKCQNLDCP 

       430        440        450        460        470        480 
SRVVNSIIYF ASKNCMNIDG LGNKIVEQLF NEKKIYDILD IYSLKYEDLQ DLEGFKEKKI 

       490        500        510        520        530        540 
NNLLNAIENT KGSELHRVIN ALGIEHIGEV ASKQICLEFG LDVINKDYDS LIALDGFGEQ 

       550        560        570        580        590        600 
MANSFIEFMR VNKNLVEKLI SIINPKVEIK KEVSENPFKN KTVVITGTMS KSRGEIKSML 

       610        620        630        640 
EDLGAKVSSS VSKKTDYVIF GEDAGSKYDK AIELGVKLLT EEEMNSLF

« Hide

References

[1]"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri."
Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E.
PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RM4018.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000361 Genomic DNA. Translation: ABV67327.1.
RefSeqYP_001489996.1. NC_009850.1.

3D structure databases

ProteinModelPortalA8ETQ3.
SMRA8ETQ3. Positions 572-644.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8ETQ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5623342.
GenomeReviewsGene locus Abu_1067 in contig CP000361_GR.
KEGGabu:Abu_1067.
PATRIC20963506. VBIArcBut20197_1054.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0272.
HOGENOMHBG620317.
OMAENVRTIR.
ProtClustDBPRK07956.

Enzyme and pathway databases

BioCycABUT367737:ABU_1067-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 3 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_ARCB4
AccessionPrimary (citable) accession number: A8ETQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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