Reviewed, UniProtKB/Swiss-Prot A8ETA7 (GUAA_ARCB4)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing]
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase
    GMP synthetase

Gene names

Name:	guaA
Ordered Locus Names:	Abu_0921

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_1000120209
Regions
	Domain	5 – 195	191	Glutamine amidotransferase type-1
	Domain	227 – 387	161	GMP-binding
	Nucleotide binding	223 – 229	7	ATP By similarity
Sites
	Active site	82	1	Nucleophile By similarity
	Active site	169	1	By similarity
	Active site	171	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ETA7-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: B6540D6033630041	FASTA	511	56,534
	10 20 30 40 50 60 MKHVPIVVLD FGSQYTQIIA RKLREAGVYS EIVPYNEPIG DIVARTPKGI ILSGGPASVY 70 80 90 100 110 120 AVDAYHPDTT IFELGLPILG ICYGMQLISQ HFGGSVIPAS HHEYGKAKLQ FEVENPIFKD 130 140 150 160 170 180 TQDGQIVWMS HGDRVENIPA GFEKIATSEN SPFAAIADIN RNIYAFQFHP EVYHSQEGSK 190 200 210 220 230 240 LLKNFAKHIC GCESTWNMGS FAKEQIKQIQ DKVGNKKVLC AVSGGVDSSV VATLLAEAIG 250 260 270 280 290 300 EQVIPVFVDN GLLRANEREQ VETMFKSRGV NLITVDASET FLSKLAGVTD PEKKRKIIGE 310 320 330 340 350 360 TFIEVFDVEA KKHSGIEFLA QGTLYTDVIE SVSVKGPSKT IKSHHNVGGL PDWMKFELIE 370 380 390 400 410 420 PLREIFKDEV RALGLELGLP KSMISRHPFP GPGLAIRIMG EVNKPDLELL RKADVIMLDV 430 440 450 460 470 480 LHSTGYYDKT WQAFTVLLNV KSVGVMGDNR TYDNTVCVRI VDATDGMTAT FAHIPHEILE 490 500 510 TISRRIINEV DGINRVVYDI SSKPPATIEW E

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000361 Genomic DNA. Translation: ABV67181.1.
RefSeq	YP_001489850.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8ETA7.
Genome annotation databases
GeneID	5624678.
GenomeReviews	Gene locus Abu_0921 in contig CP000361_GR.
KEGG	abu:Abu_0921.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DQVMEMF.
Family and domain databases
HAMAP	MF_00344. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR014729. Rossmann-like_a/b/a_fold. IPR018318. tRNA_MeTrfase-like. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF03054. tRNA_Me_trans. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_ARCB4

Accession

Primary (citable) accession number: A8ETA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)