A8ET60 (DAPA_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Abu_0874

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000060082
Regions
	Region	49 – 50	2	Pyruvate binding By similarity
Sites
	Active site	164	1	Schiff-base intermediate with substrate By similarity
	Binding site	108	1	Pyruvate By similarity
	Site	135	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ET60 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 338F1505514C960B	FASTA	295	31,931
	10 20 30 40 50 60 MDIITGSMTA LITPFKNGKV DLQKYESLIK RQIAQGINAV SPVGTTGESA TLSHKEHKEC 70 80 90 100 110 120 IEVAVATCKN SGVKVLAGAG SNATHEACDI AKFAQEVGAD GILSIAPYYN KPTQEGLYQH 130 140 150 160 170 180 YKAIANSVEL PLMLYNVPGR TGVDLLPETA IRLFDDVKNI YGIKEATGSL ERATSLMSAR 190 200 210 220 230 240 KDFVVVSGDD SVDFPMLASG ARGIISVTSN LLPNLKSKLV SSVLEGDYKT SLSIHNDLYE 250 260 270 280 290 LNKTLFCESN PIPIKAAMYL SGLLDSLEFR LPLTNPSAET MQKLEKILIK YEVIK

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM4018.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV67134.1.
RefSeq	YP_001489803.1. NC_009850.1.
3D structure databases
ProteinModelPortal	A8ET60.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ET60.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5625237.
GenomeReviews	Gene locus Abu_0874 in contig CP000361_GR.
KEGG	abu:Abu_0874.
PATRIC	20963116. VBIArcBut20197_0859.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	FMLCGGH.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	ABUT367737:ABU_0874-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_ARCB4

Accession

Primary (citable) accession number: A8ET60

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program