A8ESZ8 (URE1_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease subunit beta
EC=3.5.1.5

Alternative name(s):
Urea amidohydrolase subunit beta

Gene names

Name:	ureB
Ordered Locus Names:	Abu_0807

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity. HAMAP MF_01953
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01953.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity. HAMAP MF_01953
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.
Caution	The orthologous protein is known as the alpha subunit (UreC) in most other bacteria.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro urease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Urease subunit beta HAMAP MF_01953		PRO_1000070645
Regions
	Domain	129 – 566	438	Urease
Sites
	Active site	320	1	Proton donor By similarity
	Metal binding	134	1	Nickel 2 By similarity
	Metal binding	136	1	Nickel 2 By similarity
	Metal binding	217	1	Nickel 1; via carbamate group By similarity
	Metal binding	217	1	Nickel 2; via carbamate group By similarity
	Metal binding	246	1	Nickel 1 By similarity
	Metal binding	272	1	Nickel 1 By similarity
	Metal binding	360	1	Nickel 2 By similarity
	Binding site	219	1	Substrate By similarity
Amino acid modifications
	Modified residue	217	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ESZ8 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: BA11B373C8E5C20E	FASTA	566	60,814
	10 20 30 40 50 60 MKISKEKYAS MYGPTTGDRF RLADTTLIAK IEKDYTIYGE ESKFGGGKTV RDGMAQSPTA 70 80 90 100 110 120 VDVADLIITN AIIIDYTGIY KADIGIKDGK ILAIGKSGNP NLCDGITEGL EIGANTEILS 130 140 150 160 170 180 AEGKIITAGG IDTHIHFISP GQINEALSSG VTTMIGGGTG PNTGTNATTC TPGEWNISKM 190 200 210 220 230 240 IQSVDDLPLN FGFMGKGNSS SYEALKVQIE AGAMGLKLHE DWGSTPNAID TCLSVADDFD 250 260 270 280 290 300 VQVAIHTDTL NESGFVEATV GAFKNRTIHT FHSEGAGGGH APDIMKVAGL SNVLPSSTNP 310 320 330 340 350 360 TLPYTKNTIE EHLDMLMVCH HLSPKIPEDV SFAESRIRGK TIAAEDVLHD LGAISITSSD 370 380 390 400 410 420 SQAMGRVGEV IIRTWQVAHS MKQQRGTLEG DDEKSDNNRI KRYIAKYTIN PAIACGIDEY 430 440 450 460 470 480 VGSVEVGKMA DLVLWNRAFF GVKPEIIIKG GFIALAMMGD SNASIPTPEP NMYRLMFGSL 490 500 510 520 530 540 GRASGATSVI FTSKVASSNL KDKLGISKNV LPVKNTRNIG KANMKLNDFI GDIEIDSETY 550 560 DVKINGEPIE SNYVEKVPMA RRYFMF

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM4018.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV67072.1.
RefSeq	YP_001489741.1. NC_009850.1.
3D structure databases
ProteinModelPortal	A8ESZ8.
SMR	A8ESZ8. Positions 2-566.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ESZ8.
Protein family/group databases
MEROPS	M38.982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5623774.
GenomeReviews	Gene locus Abu_0807 in contig CP000361_GR.
KEGG	abu:Abu_0807.
PATRIC	20962982. VBIArcBut20197_0796.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0804.
HOGENOM	HBG357507.
OMA	TIHAFHT.
ProtClustDB	PRK13207.
Enzyme and pathway databases
BioCyc	ABUT367737:ABU_0807-MONOMER.
Family and domain databases
HAMAP	MF_01953. Urease_alpha. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011059. Metal-dep_hydrolase_composite. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017950. Urease_asu_CS. [Graphical view]
KO	K01428.
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
SUPFAM	SSF51338. Metalo_hydrolase. 2 hits.
TIGRFAMs	TIGR01792. Urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_ARCB4

Accession

Primary (citable) accession number: A8ESZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program