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Reviewed, UniProtKB/Swiss-Prot A8ESZ8 (URE1_ARCB4)

Last modified February 9, 2010. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Urease subunit beta
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit beta
Gene names
Name: ureB
Ordered Locus Names: Abu_0807
OrganismArcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]
Taxonomic identifier367737 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

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Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953

Cofactor

Binds 2 nickel ions per subunit By similarity. HAMAP MF_01953

Pathway

Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953

Subunit structure

Heterohexamer of 3 ureA (alpha) and 3 ureB (beta) subunits By similarity. HAMAP MF_01953

Subcellular location

Cytoplasm By similarity HAMAP MF_01953.

Post-translational modification

Carbamylation allows a single lysine to coordinate two nickel ions By similarity. HAMAP MF_01953

Sequence similarities

Belongs to the urease family.

Contains 1 urease domain.

Caution

The orthologous protein is known as the alpha subunit (ureC) in most other bacteria.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Nickel
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processurea metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnickel ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

urease activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566Urease subunit beta HAMAP MF_01953
PRO_1000070645

Regions

Domain129 – 566438Urease

Sites

Active site3201Proton donor By similarity
Metal binding1341Nickel 2 By similarity
Metal binding1361Nickel 2 By similarity
Metal binding2171Nickel 1; via carbamate group By similarity
Metal binding2171Nickel 2; via carbamate group By similarity
Metal binding2461Nickel 1 By similarity
Metal binding2721Nickel 1 By similarity
Metal binding3601Nickel 2 By similarity
Binding site2191Substrate By similarity

Amino acid modifications

Modified residue2171N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
A8ESZ8-1 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: BA11B373C8E5C20E

FASTA56660,814
        10         20         30         40         50         60 
MKISKEKYAS MYGPTTGDRF RLADTTLIAK IEKDYTIYGE ESKFGGGKTV RDGMAQSPTA 

        70         80         90        100        110        120 
VDVADLIITN AIIIDYTGIY KADIGIKDGK ILAIGKSGNP NLCDGITEGL EIGANTEILS 

       130        140        150        160        170        180 
AEGKIITAGG IDTHIHFISP GQINEALSSG VTTMIGGGTG PNTGTNATTC TPGEWNISKM 

       190        200        210        220        230        240 
IQSVDDLPLN FGFMGKGNSS SYEALKVQIE AGAMGLKLHE DWGSTPNAID TCLSVADDFD 

       250        260        270        280        290        300 
VQVAIHTDTL NESGFVEATV GAFKNRTIHT FHSEGAGGGH APDIMKVAGL SNVLPSSTNP 

       310        320        330        340        350        360 
TLPYTKNTIE EHLDMLMVCH HLSPKIPEDV SFAESRIRGK TIAAEDVLHD LGAISITSSD 

       370        380        390        400        410        420 
SQAMGRVGEV IIRTWQVAHS MKQQRGTLEG DDEKSDNNRI KRYIAKYTIN PAIACGIDEY 

       430        440        450        460        470        480 
VGSVEVGKMA DLVLWNRAFF GVKPEIIIKG GFIALAMMGD SNASIPTPEP NMYRLMFGSL 

       490        500        510        520        530        540 
GRASGATSVI FTSKVASSNL KDKLGISKNV LPVKNTRNIG KANMKLNDFI GDIEIDSETY 

       550        560 
DVKINGEPIE SNYVEKVPMA RRYFMF

« Hide

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References

[1]"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri."
Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E.
PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000361 Genomic DNA. Translation: ABV67072.1.
RefSeqYP_001489741.1.

3D structure databases

SMRA8ESZ8. Positions 2-566.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8ESZ8.

Protein family/group databases

MEROPSM38.982.

Genome annotation databases

GeneID5623774.
GenomeReviewsGene locus Abu_0807 in contig CP000361_GR.
KEGGabu:Abu_0807.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0804.
HOGENOMHBG357507.
OMASHIHFIC.

Family and domain databases

HAMAPMF_01953. Urease_alpha.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR011059. Metal-dep_hydrolase_composite.
IPR011612. Urease_alpha_N.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR017952. Urease_asu_core.
IPR017950. Urease_asu_CS.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSPR01752. UREASE.
TIGRFAMsTIGR01792. urease_alph. 1 hit.
PROSITEPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameURE1_ARCB4
AccessionPrimary (citable) accession number: A8ESZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents