A8ESW7 (SYD_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Abu_0776

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000057299

Sequences

	Sequence		Length	Mass (Da)
	A8ESW7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 0A7749D552945D5B	FASTA	589	67,053
	10 20 30 40 50 60 MRTHYCTDVT EKLIGQTVTV AGWVNSRRDH GGIIFIDLRD KSGLVQLVAD PQDCKDALAI 70 80 90 100 110 120 AETVRDEFVL IATGKVRARG EGLENPNLIT GKIEIILENL VIENRSRPMP FDINDEKVND 130 140 150 160 170 180 EIKLRNRFLE LRSKKSFDIF QLRSKATIQA RNTLDELGFL DVETPILTKS TPEGARDYLV 190 200 210 220 230 240 PSRVHAGEFY ALPQSPQLFK QLLMVAGFDK YFQIAKCFRD EDLRADRQPE FTQIDVEMSF 250 260 270 280 290 300 CNQEDVIKVA EKLIYDIFTK CGKNVPSTFR RMKYSEAMEK YGSDKPDLRF DMPLVDVIDI 310 320 330 340 350 360 FANSTNEIFA EIAKDKKNNR IKALKCKNGD NIFSKRQMKS FEDYVRKFGA KGLGYFQMKE 370 380 390 400 410 420 DGLKGPLTKF FSEADLEEII KVTELEVGDV VFFGAGAKKV VWDYMGRFRL FLANEMNIVP 430 440 450 460 470 480 KDAYEFLWVI DFPMFEVEDG RTKALHHPFT MPNVEKYDLD NIEDLEEIES IAYDIVLNGT 490 500 510 520 530 540 ELGGGSIRIH KEEIQSKVFK LMGISQEEAK EKFGFLLDAL SYGAPSHGGF ALGLDRMIML 550 560 570 580 LAGTDSIRDV IAFPKTQKAQ CLLTQAPSAV DEEQLKELSI RIRKTVVDS

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM4018.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV67041.1.
RefSeq	YP_001489710.1. NC_009850.1.
3D structure databases
ProteinModelPortal	A8ESW7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ESW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5624899.
GenomeReviews	Gene locus Abu_0776 in contig CP000361_GR.
KEGG	abu:Abu_0776.
PATRIC	20962920. VBIArcBut20197_0765.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	ABUT367737:ABU_0776-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ARCB4

Accession

Primary (citable) accession number: A8ESW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program