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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Arcobacter butzleri (strain RM4018)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi896 – 8961ZincUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciABUT367737:GHWO-518-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Abu_0519
OrganismiArcobacter butzleri (strain RM4018)
Taxonomic identifieri367737 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter
ProteomesiUP000001136 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000070884Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi367737.Abu_0519.

Structurei

3D structure databases

ProteinModelPortaliA8ES62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ES62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKESLLLP KTDFPMRGNL PQNEPVKYKQ WDEKKVYEKM KKNRVGCPSF
60 70 80 90 100
TLHDGPPYAN GNIHIGHALN KILKDIINKF HYFEGKSIRY VPGWDCHGLP
110 120 130 140 150
IEQKVEEKIG SEKKKELPKS KLRQLCRDHA TKFVEIQKDE FKKLGVIADW
160 170 180 190 200
ENPYLTMDFK FEANIYRELC AIAKQGLLIQ RSKPVYWSWA AQTALAEAEV
210 220 230 240 250
EYEDKTSPSI YVAFEMSNES KAIHGLENKK AAFVIWTTTP WTLPANTGIS
260 270 280 290 300
LHPVEMYVLT SDGYIVAKAL YSKLKEEAVI NGEIVTEFEP FMFESNIAIN
310 320 330 340 350
PLNGRTSKIV LGDHVMMDSG TGAVHTAPGH GEDDYKVGLR YNLDVIMPVD
360 370 380 390 400
AYGKYDETIV REKLFKDTNK YLGLNVFKAN ELILEELGSA LLKRVDIRHS
410 420 430 440 450
YPHCWRTHTP IIFRATKQWF ISIDDKYGNK NNTLRENALE VVENLKFYPE
460 470 480 490 500
WGRNRLKAML EGRPDWCISR QRDWGVPIAF FRNKKTDEII FDEKVLNYTA
510 520 530 540 550
MIFEQKGCDA WYDLEIKELL YPGSGLNPDD LEKTLDILDV WFDSGSTQNA
560 570 580 590 600
VLRSRNYDAG TFPADMYLEG SDQHRGWFQS SLLTTLASSE IAPYKSILTH
610 620 630 640 650
GFTVDEKGEK MSKSKGNVVA PDKVIKEYGS EILRLWVAMS DYQSDLKISD
660 670 680 690 700
NILKQNGELY RKIRNTARFL LANIDDLEEI IDVSKMGPLD KWILNKAKKV
710 720 730 740 750
FDEIEAAFNV YEFSKGLNKL NNFLVVDLSG IYLDVCKDRL YCDDKKDIHR
760 770 780 790 800
LASQSAMALI AKKLISTLAC ILTYTMDELL EFAPAFIKDG CEDIFDFKKV
810 820 830 840 850
ELPVVESSLD ESILLSAKEK FSEIKDALSK EKVIKSTLEL MLYTNSEDIL
860 870 880 890 900
ALDEVEASDW FLVSQVSKDK QNSDILGSFQ IDGKDFEVYK ATAHKCPRCW
910
KFTANAEESL CKRCEEVIK
Length:919
Mass (Da):105,337
Last modified:November 13, 2007 - v1
Checksum:i4541CC58E60DFB84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000361 Genomic DNA. Translation: ABV66786.1.
RefSeqiWP_012012317.1. NC_009850.1.

Genome annotation databases

EnsemblBacteriaiABV66786; ABV66786; Abu_0519.
GeneIDi24305643.
KEGGiabu:Abu_0519.
PATRICi20962398. VBIArcBut20197_0504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000361 Genomic DNA. Translation: ABV66786.1.
RefSeqiWP_012012317.1. NC_009850.1.

3D structure databases

ProteinModelPortaliA8ES62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi367737.Abu_0519.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV66786; ABV66786; Abu_0519.
GeneIDi24305643.
KEGGiabu:Abu_0519.
PATRICi20962398. VBIArcBut20197_0504.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciABUT367737:GHWO-518-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM4018.

Entry informationi

Entry nameiSYI_ARCB4
AccessioniPrimary (citable) accession number: A8ES62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: July 22, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.