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A8ES62

- SYI_ARCB4

UniProt

A8ES62 - SYI_ARCB4

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Arcobacter butzleri (strain RM4018)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi896 – 8961ZincUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciABUT367737:GHWO-518-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Abu_0519
OrganismiArcobacter butzleri (strain RM4018)
Taxonomic identifieri367737 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter
ProteomesiUP000001136: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000070884Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi367737.Abu_0519.

Structurei

3D structure databases

ProteinModelPortaliA8ES62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ES62-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKESLLLP KTDFPMRGNL PQNEPVKYKQ WDEKKVYEKM KKNRVGCPSF
60 70 80 90 100
TLHDGPPYAN GNIHIGHALN KILKDIINKF HYFEGKSIRY VPGWDCHGLP
110 120 130 140 150
IEQKVEEKIG SEKKKELPKS KLRQLCRDHA TKFVEIQKDE FKKLGVIADW
160 170 180 190 200
ENPYLTMDFK FEANIYRELC AIAKQGLLIQ RSKPVYWSWA AQTALAEAEV
210 220 230 240 250
EYEDKTSPSI YVAFEMSNES KAIHGLENKK AAFVIWTTTP WTLPANTGIS
260 270 280 290 300
LHPVEMYVLT SDGYIVAKAL YSKLKEEAVI NGEIVTEFEP FMFESNIAIN
310 320 330 340 350
PLNGRTSKIV LGDHVMMDSG TGAVHTAPGH GEDDYKVGLR YNLDVIMPVD
360 370 380 390 400
AYGKYDETIV REKLFKDTNK YLGLNVFKAN ELILEELGSA LLKRVDIRHS
410 420 430 440 450
YPHCWRTHTP IIFRATKQWF ISIDDKYGNK NNTLRENALE VVENLKFYPE
460 470 480 490 500
WGRNRLKAML EGRPDWCISR QRDWGVPIAF FRNKKTDEII FDEKVLNYTA
510 520 530 540 550
MIFEQKGCDA WYDLEIKELL YPGSGLNPDD LEKTLDILDV WFDSGSTQNA
560 570 580 590 600
VLRSRNYDAG TFPADMYLEG SDQHRGWFQS SLLTTLASSE IAPYKSILTH
610 620 630 640 650
GFTVDEKGEK MSKSKGNVVA PDKVIKEYGS EILRLWVAMS DYQSDLKISD
660 670 680 690 700
NILKQNGELY RKIRNTARFL LANIDDLEEI IDVSKMGPLD KWILNKAKKV
710 720 730 740 750
FDEIEAAFNV YEFSKGLNKL NNFLVVDLSG IYLDVCKDRL YCDDKKDIHR
760 770 780 790 800
LASQSAMALI AKKLISTLAC ILTYTMDELL EFAPAFIKDG CEDIFDFKKV
810 820 830 840 850
ELPVVESSLD ESILLSAKEK FSEIKDALSK EKVIKSTLEL MLYTNSEDIL
860 870 880 890 900
ALDEVEASDW FLVSQVSKDK QNSDILGSFQ IDGKDFEVYK ATAHKCPRCW
910
KFTANAEESL CKRCEEVIK
Length:919
Mass (Da):105,337
Last modified:November 13, 2007 - v1
Checksum:i4541CC58E60DFB84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000361 Genomic DNA. Translation: ABV66786.1.
RefSeqiYP_001489455.1. NC_009850.1.

Genome annotation databases

EnsemblBacteriaiABV66786; ABV66786; Abu_0519.
GeneIDi5625338.
KEGGiabu:Abu_0519.
PATRICi20962398. VBIArcBut20197_0504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000361 Genomic DNA. Translation: ABV66786.1 .
RefSeqi YP_001489455.1. NC_009850.1.

3D structure databases

ProteinModelPortali A8ES62.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 367737.Abu_0519.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV66786 ; ABV66786 ; Abu_0519 .
GeneIDi 5625338.
KEGGi abu:Abu_0519.
PATRICi 20962398. VBIArcBut20197_0504.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ABUT367737:GHWO-518-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM4018.

Entry informationi

Entry nameiSYI_ARCB4
AccessioniPrimary (citable) accession number: A8ES62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3