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A8ES62

- SYI_ARCB4

UniProt

A8ES62 - SYI_ARCB4

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Abu_0519
Organism
Arcobacter butzleri (strain RM4018)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylate By similarity
Binding sitei613 – 6131ATP By similarity
Metal bindingi896 – 8961Zinc By similarity
Metal bindingi899 – 8991Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi914 – 9141Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciABUT367737:GHWO-518-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Abu_0519
OrganismiArcobacter butzleri (strain RM4018)
Taxonomic identifieri367737 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter
ProteomesiUP000001136: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligaseUniRule annotation
PRO_1000070884Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi367737.Abu_0519.

Structurei

3D structure databases

ProteinModelPortaliA8ES62.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi610 – 6145"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ES62-1 [UniParc]FASTAAdd to Basket

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MDYKESLLLP KTDFPMRGNL PQNEPVKYKQ WDEKKVYEKM KKNRVGCPSF    50
TLHDGPPYAN GNIHIGHALN KILKDIINKF HYFEGKSIRY VPGWDCHGLP 100
IEQKVEEKIG SEKKKELPKS KLRQLCRDHA TKFVEIQKDE FKKLGVIADW 150
ENPYLTMDFK FEANIYRELC AIAKQGLLIQ RSKPVYWSWA AQTALAEAEV 200
EYEDKTSPSI YVAFEMSNES KAIHGLENKK AAFVIWTTTP WTLPANTGIS 250
LHPVEMYVLT SDGYIVAKAL YSKLKEEAVI NGEIVTEFEP FMFESNIAIN 300
PLNGRTSKIV LGDHVMMDSG TGAVHTAPGH GEDDYKVGLR YNLDVIMPVD 350
AYGKYDETIV REKLFKDTNK YLGLNVFKAN ELILEELGSA LLKRVDIRHS 400
YPHCWRTHTP IIFRATKQWF ISIDDKYGNK NNTLRENALE VVENLKFYPE 450
WGRNRLKAML EGRPDWCISR QRDWGVPIAF FRNKKTDEII FDEKVLNYTA 500
MIFEQKGCDA WYDLEIKELL YPGSGLNPDD LEKTLDILDV WFDSGSTQNA 550
VLRSRNYDAG TFPADMYLEG SDQHRGWFQS SLLTTLASSE IAPYKSILTH 600
GFTVDEKGEK MSKSKGNVVA PDKVIKEYGS EILRLWVAMS DYQSDLKISD 650
NILKQNGELY RKIRNTARFL LANIDDLEEI IDVSKMGPLD KWILNKAKKV 700
FDEIEAAFNV YEFSKGLNKL NNFLVVDLSG IYLDVCKDRL YCDDKKDIHR 750
LASQSAMALI AKKLISTLAC ILTYTMDELL EFAPAFIKDG CEDIFDFKKV 800
ELPVVESSLD ESILLSAKEK FSEIKDALSK EKVIKSTLEL MLYTNSEDIL 850
ALDEVEASDW FLVSQVSKDK QNSDILGSFQ IDGKDFEVYK ATAHKCPRCW 900
KFTANAEESL CKRCEEVIK 919
Length:919
Mass (Da):105,337
Last modified:November 13, 2007 - v1
Checksum:i4541CC58E60DFB84
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000361 Genomic DNA. Translation: ABV66786.1.
RefSeqiYP_001489455.1. NC_009850.1.

Genome annotation databases

EnsemblBacteriaiABV66786; ABV66786; Abu_0519.
GeneIDi5625338.
KEGGiabu:Abu_0519.
PATRICi20962398. VBIArcBut20197_0504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000361 Genomic DNA. Translation: ABV66786.1 .
RefSeqi YP_001489455.1. NC_009850.1.

3D structure databases

ProteinModelPortali A8ES62.
ModBasei Search...

Protein-protein interaction databases

STRINGi 367737.Abu_0519.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV66786 ; ABV66786 ; Abu_0519 .
GeneIDi 5625338.
KEGGi abu:Abu_0519.
PATRICi 20962398. VBIArcBut20197_0504.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ABUT367737:GHWO-518-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM4018.

Entry informationi

Entry nameiSYI_ARCB4
AccessioniPrimary (citable) accession number: A8ES62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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