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A8ERV3 (SYL_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Abu_0402
OrganismArcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]
Taxonomic identifier367737 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter

Protein attributes

Sequence length821 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 821821Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000057341

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif586 – 5905"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5891ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8ERV3 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: C1F899A1C28CB234

FASTA82194,469
        10         20         30         40         50         60 
MEYISKDIEK KWQNFWSENQ SFEPSSSKTK EKKYILSMFP YPSGRIHMGH VRNYCIGDAF 

        70         80         90        100        110        120 
ARHFRKSDFN VLHPIGWDSF GMPAENAAIK HKLHPKKWTY ENIDYMRDEL KSLGLSFSKN 

       130        140        150        160        170        180 
REFATSDELY TKWEQEFIIK MYEAGIIYRK SATVNWCPHD QTVLANEQLE EGCCWRCGTE 

       190        200        210        220        230        240 
VVQKEMPGYY IGITKYAQEL LDDLEKLKED WPSQVLTMQE NWIGRSEGLE FKFDLSKESR 

       250        260        270        280        290        300 
AKLERAFTKY FVFTTRPDTI YGVSYSALAP EHPIVKYIVE KNLLPEKKIK AIKAMQKIPE 

       310        320        330        340        350        360 
RDRATQEKEG IDLEIEVMHP LTGKTIPVWV ANFVLSSYGG GAVMAVPAHD QRDFEFAKKY 

       370        380        390        400        410        420 
NLPIKQVIVG PDGIIENQTE AYTAEGRLIE SENFTGVTNI EAKKAIIYHF EQNSFGIKKV 

       430        440        450        460        470        480 
NYKLRDWGVS RQRYWGAPIP FIHCEKCGLV PEKIENLPVA LPEDVEITGE GNPLDTHPTW 

       490        500        510        520        530        540 
KHCTCPKCGE KATRETDTLD TFVQSSWYFL RYATDNKKWN EVGISKEDSD YWMDVDQYIG 

       550        560        570        580        590        600 
GIEHAILHLL YARFFTKVLR DLGYTNSSEP FKKLLTQGMV LKDGAKMSKS KGNVVDPDLI 

       610        620        630        640        650        660 
IDKYGADTAR LFILFAAPPT KELEWNDSAV EGAFRFIKKF FERAENVNQN GLDNFKSIDH 

       670        680        690        700        710        720 
SALSKEEKEA RKKVYEALLK SNEVFTKTYT FNTLIASCME ALNALQTQKN DSIWAEGYYI 

       730        740        750        760        770        780 
LTNILEPIIP HACWELSKKL FDLKNFDGKI ELKEEVFALE SIILAVTVNG KKRCEIEVAP 

       790        800        810        820 
DTSKDEILVK AKIASAKWLE NSEILKEIVV PNKLVNFVIK G 

« Hide

References

[1]"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri."
Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E.
PLoS ONE 2:E1358-E1358(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RM4018.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000361 Genomic DNA. Translation: ABV66677.1.
RefSeqYP_001489346.1. NC_009850.1.

3D structure databases

ProteinModelPortalA8ERV3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING367737.Abu_0402.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV66677; ABV66677; Abu_0402.
GeneID5624639.
KEGGabu:Abu_0402.
PATRIC20962162. VBIArcBut20197_0394.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycABUT367737:GHWO-401-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ARCB4
AccessionPrimary (citable) accession number: A8ERV3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries