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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Arcobacter butzleri (strain RM4018)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei589 – 5891ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciABUT367737:GHWO-401-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Abu_0402
OrganismiArcobacter butzleri (strain RM4018)
Taxonomic identifieri367737 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeArcobacter
ProteomesiUP000001136: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 821821Leucine--tRNA ligasePRO_1000057341Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi367737.Abu_0402.

Structurei

3D structure databases

ProteinModelPortaliA8ERV3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionAdd
BLAST
Motifi586 – 5905"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ERV3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYISKDIEK KWQNFWSENQ SFEPSSSKTK EKKYILSMFP YPSGRIHMGH
60 70 80 90 100
VRNYCIGDAF ARHFRKSDFN VLHPIGWDSF GMPAENAAIK HKLHPKKWTY
110 120 130 140 150
ENIDYMRDEL KSLGLSFSKN REFATSDELY TKWEQEFIIK MYEAGIIYRK
160 170 180 190 200
SATVNWCPHD QTVLANEQLE EGCCWRCGTE VVQKEMPGYY IGITKYAQEL
210 220 230 240 250
LDDLEKLKED WPSQVLTMQE NWIGRSEGLE FKFDLSKESR AKLERAFTKY
260 270 280 290 300
FVFTTRPDTI YGVSYSALAP EHPIVKYIVE KNLLPEKKIK AIKAMQKIPE
310 320 330 340 350
RDRATQEKEG IDLEIEVMHP LTGKTIPVWV ANFVLSSYGG GAVMAVPAHD
360 370 380 390 400
QRDFEFAKKY NLPIKQVIVG PDGIIENQTE AYTAEGRLIE SENFTGVTNI
410 420 430 440 450
EAKKAIIYHF EQNSFGIKKV NYKLRDWGVS RQRYWGAPIP FIHCEKCGLV
460 470 480 490 500
PEKIENLPVA LPEDVEITGE GNPLDTHPTW KHCTCPKCGE KATRETDTLD
510 520 530 540 550
TFVQSSWYFL RYATDNKKWN EVGISKEDSD YWMDVDQYIG GIEHAILHLL
560 570 580 590 600
YARFFTKVLR DLGYTNSSEP FKKLLTQGMV LKDGAKMSKS KGNVVDPDLI
610 620 630 640 650
IDKYGADTAR LFILFAAPPT KELEWNDSAV EGAFRFIKKF FERAENVNQN
660 670 680 690 700
GLDNFKSIDH SALSKEEKEA RKKVYEALLK SNEVFTKTYT FNTLIASCME
710 720 730 740 750
ALNALQTQKN DSIWAEGYYI LTNILEPIIP HACWELSKKL FDLKNFDGKI
760 770 780 790 800
ELKEEVFALE SIILAVTVNG KKRCEIEVAP DTSKDEILVK AKIASAKWLE
810 820
NSEILKEIVV PNKLVNFVIK G
Length:821
Mass (Da):94,469
Last modified:November 13, 2007 - v1
Checksum:iC1F899A1C28CB234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000361 Genomic DNA. Translation: ABV66677.1.
RefSeqiYP_001489346.1. NC_009850.1.

Genome annotation databases

EnsemblBacteriaiABV66677; ABV66677; Abu_0402.
GeneIDi5624639.
KEGGiabu:Abu_0402.
PATRICi20962162. VBIArcBut20197_0394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000361 Genomic DNA. Translation: ABV66677.1.
RefSeqiYP_001489346.1. NC_009850.1.

3D structure databases

ProteinModelPortaliA8ERV3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi367737.Abu_0402.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV66677; ABV66677; Abu_0402.
GeneIDi5624639.
KEGGiabu:Abu_0402.
PATRICi20962162. VBIArcBut20197_0394.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciABUT367737:GHWO-401-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM4018.

Entry informationi

Entry nameiSYL_ARCB4
AccessioniPrimary (citable) accession number: A8ERV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: January 7, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.