A8ERT9 (SYP_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Abu_0388

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Proline--tRNA ligase HAMAP MF_01569		PRO_1000069119

Sequences

	Sequence		Length	Mass (Da)
	A8ERT9 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: D38B375B4D0C812A	FASTA	568	63,315
	10 20 30 40 50 60 MKFSKMFIPT TKETPNDATL PSHQYLVRGG FIAQTGAGIY DFMPLGKIVL EKIRAIVKEE 70 80 90 100 110 120 MDEAGANEVQ FGFVTPLTLW QESGRATTMG AEMLRFKDRK NGEFVLSPTN EEAVVNMVKN 130 140 150 160 170 180 RITSYKDLPL HLYQINTKFR DEARPRFGLM RGREFLMKDG YSFHSSEEDL VREFNLMETT 190 200 210 220 230 240 YKKIYTKLGL DFRVVAADSG AIGGSGSKEF HVIADSGEDT LVVCDSCDYG ANIEAAIRKP 250 260 270 280 290 300 KTYSFERKSD SKKIHTPNTK TIEEVANFLN ISKEQTIKAV IKKAIYEEKT QIVIFFVRGS 310 320 330 340 350 360 DELEETKACN AVNALELIDA SEDDIKEAGL VAGYCGLFNL PSNINFIIDL ELKDEIGLAC 370 380 390 400 410 420 GANEEDYHLV NTDLSTLKDV KYYDLIAVQE GDICACCGGK LSYTKGIEAG HIFQLGTKYS 430 440 450 460 470 480 SAMNANFLDE NGKAKPFIMG CYGIGVSRLV AAVIEQNHDD KGCIWTKATA PFMVDIIVSN 490 500 510 520 530 540 SKKEEEAKVG EELYSKLKQA GISTILDDRI NARFGFKMSD FELLGFPYAV VIGKKLEDGL 550 560 VEIVDRKTLE KIDVKVDEVI SKILELVK

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM4018.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV66663.1.
RefSeq	YP_001489332.1. NC_009850.1.
3D structure databases
ProteinModelPortal	A8ERT9.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ERT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5624714.
GenomeReviews	Gene locus Abu_0388 in contig CP000361_GR.
KEGG	abu:Abu_0388.
PATRIC	20962136. VBIArcBut20197_0381.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	ABUT367737:ABU_0388-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ARCB4

Accession

Primary (citable) accession number: A8ERT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program