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A8ERP8 (NRFA_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome c-552
EC=1.7.2.2
Alternative name(s):
Ammonia-forming cytochrome c nitrite reductase
Short name=Cytochrome c nitrite reductase
Gene names
Name:nrfA
Ordered Locus Names:Abu_0347
OrganismArcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]
Taxonomic identifier367737 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity. HAMAP MF_01182

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity. HAMAP MF_01182

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity HAMAP MF_01182.

Sequence similarities

Belongs to the cytochrome c-552 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 496473Cytochrome c-552 HAMAP MF_01182
PRO_1000085447

Sites

Metal binding1001Iron (heme 3 axial ligand) By similarity
Metal binding1321Iron (heme 1 axial ligand) By similarity
Metal binding1701Iron (heme 2 axial ligand) By similarity
Metal binding2141Iron (heme 3 axial ligand) By similarity
Metal binding2161Calcium By similarity
Metal binding2171Calcium; via carbonyl oxygen By similarity
Metal binding2691Calcium; via carbonyl oxygen By similarity
Metal binding2711Calcium By similarity
Metal binding2831Iron (heme 5 axial ligand) By similarity
Metal binding2941Iron (heme 4 axial ligand) By similarity
Metal binding3081Iron (heme 2 axial ligand) By similarity
Metal binding3251Iron (heme 5 axial ligand) By similarity
Metal binding4001Iron (heme 4 axial ligand) By similarity
Binding site1281Heme 1 (covalent) By similarity
Binding site1311Heme 1 (covalent) By similarity
Binding site1661Heme 2 (covalent) By similarity
Binding site1691Heme 2 (covalent) By similarity
Binding site2101Heme 3 (covalent) By similarity
Binding site2131Heme 3 (covalent) By similarity
Binding site2171Substrate By similarity
Binding site2721Substrate By similarity
Binding site2901Heme 4 (covalent) By similarity
Binding site2931Heme 4 (covalent) By similarity
Binding site3211Heme 5 (covalent) By similarity
Binding site3241Heme 5 (covalent) By similarity

Sequences

Sequence LengthMass (Da)Tools
A8ERP8 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: DE03F5334191555F

FASTA49656,273
        10         20         30         40         50         60 
MKKYKFLFAI SIIAIGLMTV LLASINEKKE EKENLLSVPK IEKWETKNEE FRKFYPREFD 

        70         80         90        100        110        120 
SWKQTKNSDQ IDDMLKLHPE MVVLWAGYAF SKDYNAPRGH FYAIDDVSNS LRTGAPTDKE 

       130        140        150        160        170        180 
SGPLPSACWT CKSPDVPRIM HEQGNNEYFT GKWAKYGADI VNPIGCVDCH NPETMELQVG 

       190        200        210        220        230        240 
RSYLNDALKA EGKSPTLATA TQQDMRTLVC AQCHVEYYFK KTPLENGKTA MAVTLPWANG 

       250        260        270        280        290        300 
TTVEDMEKYY DTIEFSDWVH QVSKTPMIKA QHPEYETWKT GAHGRNNVSC ADCHMPYTQE 

       310        320        330        340        350        360 
GGIKYTDHKI GNPLENMDKT CMNCHRVSEK SLLANIQDKK ARKDDLNQKA MEQIVAAHLE 

       370        380        390        400        410        420 
AGKAWEVGAT PEEMKDILTD IRHAQWRWDF AAASHSAFFH APEETLKTLG TAIEKAGNAR 

       430        440        450        460        470        480 
IKLAKVLAKH GVTDYKAPTI TDKKQAQELI GLPMGKLIEE KKQFTNGLLK DWKNEAEQKG 

       490 
LYNPKSREGV ETKTSY

« Hide

References

[1]"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri."
Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E.
PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RM4018.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000361 Genomic DNA. Translation: ABV66622.1.
RefSeqYP_001489291.1. NC_009850.1.

3D structure databases

ProteinModelPortalA8ERP8.
SMRA8ERP8. Positions 44-496.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8ERP8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5624529.
GenomeReviewsGene locus Abu_0347 in contig CP000361_GR.
KEGGabu:Abu_0347.
PATRIC20962054. VBIArcBut20197_0340.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3303.
HOGENOMHBG488281.
OMARDINSAC.
ProtClustDBPRK11125.

Enzyme and pathway databases

BioCycABUT367737:ABU_0347-MONOMER.

Family and domain databases

HAMAPMF_01182. Cytochrom_C552.
[Tree]
InterProIPR023155. Cyt_c-552/4.
IPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
Gene3DG3DSA:1.10.710.10. Cytochrome_c-552/4. 2 hits.
KOK03385.
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNRFA_ARCB4
AccessionPrimary (citable) accession number: A8ERP8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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