Reviewed, UniProtKB/Swiss-Prot A8ERE6 (SYC_ARCB4)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Abu_0245

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000332786
Regions
	Motif	32 – 42	11	"HIGH" region HAMAP MF_00041
	Motif	271 – 275	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	30	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Metal binding	243	1	Zinc By similarity
	Binding site	274	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ERE6-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 38087A0920A24301	FASTA	467	53,425
	10 20 30 40 50 60 MNTLHIFDSV KKEKVEFKPI QEGKVKIYVC GPTVYDDSHL GHARSAIAFD LLHRVLKVND 70 80 90 100 110 120 YEVTMTKNFT DIDDKIIKKM YETNKTLENI TNQYINAYKA DMKALNILDN TIEPKATENL 130 140 150 160 170 180 EVMKEMISNL ISKDVAYKTS DSVYFDTSKD NLYGTLSHKS NDENSQARVE ENQEKRNSAD 190 200 210 220 230 240 FALWKFEKAN DVSFDAPFGK GRPGWHIECS AMIEKHLAYK DSPYQIDIHA GGADLLFPHH 250 260 270 280 290 300 ENEAAQTRCS SGQNLAKYWM HNGFVNINGE KMSKSLGNSF FLKDVLKSYS GEVIRFYLMS 310 320 330 340 350 360 THYRADLSFN EEDLIASKKR LDKIYRLKKR VYGIEDSSVN KKFKEDILNA LNDDINTSIA 370 380 390 400 410 420 LSVIDEMINS ANDKLDSNPK DKNLKKELIS NINFIEEVLG IGGNDAYAYF QFGINESTKE 430 440 450 460 KIESLILKRN EAKKTKDFQT ADKLRDELSS MDISLMDTVN GTVWEKL

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV66520.1.
RefSeq	YP_001489189.1.
3D structure databases
SMR	A8ERE6. Positions 4-405.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ERE6.
Genome annotation databases
GeneID	5623590.
GenomeReviews	Gene locus Abu_0245 in contig CP000361_GR.
KEGG	abu:Abu_0245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041_B. Cys_tRNA_synth_B. [Tree]
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ARCB4

Accession

Primary (citable) accession number: A8ERE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	November 13, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)