Reviewed, UniProtKB/Swiss-Prot A8ER43 (PURA_ARCB4)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	Abu_0132

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000321792
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	136	1	By similarity
	Active site	143	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ER43-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F5BB14929BC3351C	FASTA	416	45,708
	10 20 30 40 50 60 MKADVIVGIQ WGDEGKGKIV DMLAQKYDMV CRSQGGHNAG HTIWVDGVRY ALQLIPSGIL 70 80 90 100 110 120 NPKAVNVIGN GVVVSPLNII KEMSQFSSLE GRLFISDKAH LNLPFHALID QAKERLKGDK 130 140 150 160 170 180 AIGTTGKGIG PTYAEKVSRT GFRMGELLNP IKLCDDILEY FKQNRSIFDV LDISTPNKDE 190 200 210 220 230 240 LLAELNEYKE KLSPFITNTT NLVWKAIDEN KRILLEGAQG TMLDIDHGTY PYVTSSSTVS 250 260 270 280 290 300 AGSCTGLGIN PKDIGVITGI VKAYCTRVGN GPFPTEDFTE AGKTIGEVGK EFGTVTGRKR 310 320 330 340 350 360 RCGWFDAVAV RYASRLNGCD QLALMKLDVL DGFSKIKVCV AYELDGERID YMPSDMQNVK 370 380 390 400 410 PIYEEIDGWD SVVGIQDFDK LPVNAKKYIE KIEEITSVKV GIISTSPERA DTIIRG

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed: 18159241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV66417.1.
RefSeq	YP_001489086.1.
3D structure databases
SMR	A8ER43. Positions 2-415.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ER43.
Genome annotation databases
GeneID	5625048.
GenomeReviews	Gene locus Abu_0132 in contig CP000361_GR.
KEGG	abu:Abu_0132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0104.
HOGENOM	HBG658237.
OMA	IPVCVAY.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ARCB4

Accession

Primary (citable) accession number: A8ER43

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 13, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)