A8EQW9 (SYE1_ARCB4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Abu_0058

Organism

Arcobacter butzleri (strain RM4018) [Complete proteome] [HAMAP]

Taxonomic identifier

367737 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Arcobacter › Arcobacter butzleri

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamate--tRNA ligase 1 HAMAP-Rule MF_00022		PRO_0000330951
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8EQW9 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 802F588585136A69	FASTA	469	53,551
	10 20 30 40 50 60 MAITRFAPSP TGYLHIGGLR TSLYSYLWAR KTGGEFRLRI EDTDLARNSE EAMKAIIDAF 70 80 90 100 110 120 DWVGLNYDGE VFYQSKRTDI YKQYIDKLLE SGNAYKCYMS KEELDALRAA QEAAKQTPRY 130 140 150 160 170 180 DGTWRPEPGK ELPPVPAGVE PVIRIKAPTT GTIEFDDGVK GHMKFDANQV DDYVIARSNG 190 200 210 220 230 240 MPTYNFVVAI DDALMGMTDV IRGDDHLSNT PKQIVVYNAL GFKVPKFYHV PMINNPEGKK 250 260 270 280 290 300 LSKRDGAMDV MDYKRLGYLP EALLNFLVRL GWSNGDQEIF SMKEMLELFD PSNINKSASS 310 320 330 340 350 360 YNGEKLLWLN SEYIKAVSNE RLIEELKFFD LDLSNYPKKN EILDLAKQRA QTLVELKKSI 370 380 390 400 410 420 TDIIDIPTSY EESGVKKFIK EDTKELLEKY LLLLESNKNS LDSVEKIEEF TKPFINDNGL 430 440 450 460 KFPQLFQPIR IALTGGTQAP SVYDIIFILG YDEIFKRINE ALKRNFQNT

References

[1]

"The complete genome sequence and analysis of the Epsilonproteobacterium Arcobacter butzleri." Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Woesten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. PLoS ONE 2:E1358-E1358(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM4018.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000361 Genomic DNA. Translation: ABV66343.1.
RefSeq	YP_001489012.1. NC_009850.1.
3D structure databases
ProteinModelPortal	A8EQW9.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	367737.Abu_0058.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV66343; ABV66343; Abu_0058.
GeneID	5625145.
KEGG	abu:Abu_0058.
PATRIC	20961474. VBIArcBut20197_0058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	IQECIND.
OrthoDB	EOG6DRPF7.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	ABUT367737:GHWO-58-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_bac/mito. IPR000924. Glu/Gln-tRNA-synth. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. SSF48163. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_ARCB4

Accession

Primary (citable) accession number: A8EQW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	November 13, 2007
Last modified:	February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program