Reviewed, UniProtKB/Swiss-Prot A8E657 (AASS_BOVIN)

Names and origin

Protein names

Recommended name:
    Alpha-aminoadipic semialdehyde synthase, mitochondrial
Alternative name(s):
    LKR/SDH
Including the following 2 domains:
    1- Recommended name:
            Lysine ketoglutarate reductase
                Short name=LKR
                Short name=LOR
              EC=1.5.1.8
    2- Recommended name:
            Saccharopine dehydrogenase
                Short name=SDH
              EC=1.5.1.9

Gene names

Name:

AASS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	926 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively By similarity. UniProtKB Q9UDR5
Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH. UniProtKB Q9UDR5 N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-glutamate + 2-aminoadipate 6-semialdehyde + NADH. UniProtKB Q9UDR5
Pathway	Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 1/6. Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6.
Subunit structure	Homodimer By similarity. UniProtKB Q9UDR5
Subcellular location	Mitochondrion By similarity. UniProtKB Q9UDR5
Sequence similarities	In the N-terminal section; belongs to the AlaDH/PNT family. In the C-terminal section; belongs to the saccharopine dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity Inferred from electronic annotation. Source: EC saccharopine dehydrogenase (NADP+, L-lysine-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Mitochondrion By similarity UniProtKB Q9UDR5
	Chain	33 – 926	894	Alpha-aminoadipic semialdehyde synthase, mitochondrial UniProtKB Q9UDR5		PRO_0000315867
Regions
	Region	33 – 455	423	Lysine-ketoglutarate reductase By similarity UniProtKB Q9UDR5
	Region	477 – 926	450	Saccharopine dehydrogenase By similarity UniProtKB Q9UDR5

Sequences

	Sequence		Length	Mass (Da)
	A8E657-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 81A826B1856D9604	FASTA	926	102,084
	10 20 30 40 50 60 MLRVSRTKLG RLSPSLSRGL HHKAVMALRR EDVNAWERRA PLAPRHVKGI TNLGYKVLIQ 70 80 90 100 110 120 PSNRRAIHDK EYVKAGGILQ EDISEACLIL GVKRPPEEKL MPKKTYAFFS HTIKAQEANM 130 140 150 160 170 180 GLLDEILKQE IRLIDYEKMV DHRGIRVVAF GQWAGVAGII NILHGMGLRL LALGHHTPFM 190 200 210 220 230 240 HIGMAHNYRN SGQAVQAVRD AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQEIFNELPC 250 260 270 280 290 300 EYVEPHELKE VSQNGDLRKV YGTVLSRHHH LVRKTDGVYD PVEYDKYPER YISRFNTDIA 310 320 330 340 350 360 PYTTCLINGI YWEQNTPRLL TRQDAQSLLA PGKSPVAGVE GCPALPHKLV AICDISADTG 370 380 390 400 410 420 GSIEFMTECT TIERPFCMYD ADQHIIHDSV EGSGILMCSI DNLPAQLPIE STEYFGDMLY 430 440 450 460 470 480 PYVEEMILSD ATQPLESQNF SPVVRDAVIA SNGMLSNKYK YIQKLRENRE HAQSLSMGTK 490 500 510 520 530 540 KKVLVLGSGY VSEPVLEYLL RDDSIEITVG SDMKNQIEQL GKKYNINPVS LHVGKQEEKL 550 560 570 580 590 600 SSLVATQDLV ISLLPYVLHP LVAKACIASK VNMITASYIT PALKELEKSV EDAGITVIGE 610 620 630 640 650 660 LGLDPGLDHM LAMETIDKAK EVGATIESYV SYCGGLPAPE CSDNPLRYKF SWSPVGVLMN 670 680 690 700 710 720 IMQPATYLLN GKVVNAVGGV SFLDSVTPMD YFPGLNLEGY PNRDSTKYAE IYGIPSAHTL 730 740 750 760 770 780 LRGTLRYKGY AKALSGFVKL GLINRDAFPA LQPDANPLTW KELLCDLVGI SSSSKCDVLK 790 800 810 820 830 840 EAVFKKLGGD TTQLEALEWL GLLGDEQVPQ AESLVDALSK HLAVKLSYGP GEKDMIVMRD 850 860 870 880 890 900 SFGIRHPSGH LENKTIDLVV YGDVNGFSAM AKTVGLPTAM AAKMLLDGEI QAKGLMGPFS 910 920 KEIYGPILER IKAEGIMYTT QSTIKL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC153851 mRNA. Translation: AAI53852.1.
IPI	IPI00871114.
RefSeq	NP_001103267.1.
UniGene	Bt.39765
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000012128. Bos taurus. [Contig view]
GeneID	520865.
KEGG	bta:520865.
Enzyme and pathway databases
BRENDA	1.5.1.8. 251. 1.5.1.9. 251.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR007886. Ala_DH/PNT_N. IPR005097. Saccharopine_DH. [Graphical view]
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. PF03435. Saccharop_dh. 1 hit. [Graphical view]
PROSITE	PS00836. ALADH_PNT_1. False negative. PS00837. ALADH_PNT_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AASS_BOVIN

Accession

Primary (citable) accession number: A8E657

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)