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Protein

N-alpha-acetyltransferase 60

Gene

naa60

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol. Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences.By similarity

Catalytic activityi

Acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-[transmembrane protein] + CoA.By similarity
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38SubstrateBy similarity1
Active sitei97By similarity1
Binding sitei99Substrate; via carbonyl oxygenBy similarity1
Active sitei138By similarity1
Binding sitei143Acetyl-CoABy similarity1
Binding sitei165SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Chromatin regulator, Transferase
Biological processChromosome partition

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase 60 (EC:2.3.1.259By similarity)
Alternative name(s):
Histone acetyltransferase type B protein 4By similarity (EC:2.3.1.48By similarity)
Short name:
HAT4By similarity
N-acetyltransferase 15
N-alpha-acetyltransferase F
Short name:
NatF
Gene namesi
Name:naa60
Synonyms:nat15
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-941561 naa60

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 192CytoplasmicBy similarityAdd BLAST192
Intramembranei193 – 236HelicalBy similarityAdd BLAST44
Topological domaini237 – 242CytoplasmicBy similarity6

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003215701 – 242N-alpha-acetyltransferase 60Add BLAST242

Proteomic databases

PaxDbiA8E5V7
PRIDEiA8E5V7

Expressioni

Gene expression databases

BgeeiENSXETG00000031356

Interactioni

Subunit structurei

Monomer and homodimer; monomer in presence of substrate and homodimer in its absence.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000063481

Structurei

3D structure databases

ProteinModelPortaliA8E5V7
SMRiA8E5V7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 182N-acetyltransferasePROSITE-ProRule annotationAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 103Acetyl-CoA bindingBy similarity3
Regioni109 – 114Acetyl-CoA bindingBy similarity6
Regioni150 – 153Acetyl-CoA bindingBy similarity4
Regioni162 – 173Required for homodimerizationBy similarityAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IQK0 Eukaryota
ENOG410Y94A LUCA
GeneTreeiENSGT00390000008314
HOVERGENiHBG061680
InParanoidiA8E5V7
KOiK21121
OMAiSIPQRLY
OrthoDBiEOG091G0L3L
TreeFamiTF323980

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

A8E5V7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEERPAAL ADTILRFLCH DDIDTVKELC ADWFPIEYPD SWYRDITSNK
60 70 80 90 100
KFFSLAATYN GQIVGMIVAE IKGRTKVHKE DGDILASSFS GDTQVAYILS
110 120 130 140 150
LGVVKEFRKQ GIGSLLLESL KSHISSTAQD HCKALYLHVL TTNSNAIRFY
160 170 180 190 200
ENRHFHQHHY LPYYYSIRGV LQDAYTYVLY LNGGHPPWTV MDYLQHLGSA
210 220 230 240
LAGFSPCTLP QRIYRQAHTL LRSLLPWSSI SAKSGIEYSR TM
Length:242
Mass (Da):27,575
Last modified:November 13, 2007 - v1
Checksum:iD9F47EBE43CC3FDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153734 mRNA Translation: AAI53735.1
RefSeqiNP_001106444.1, NM_001112973.1
XP_012825451.1, XM_012969997.2
UniGeneiStr.42853

Genome annotation databases

EnsembliENSXETT00000060272; ENSXETP00000063481; ENSXETG00000031356
GeneIDi100127619
KEGGixtr:100127619

Similar proteinsi

Entry informationi

Entry nameiNAA60_XENTR
AccessioniPrimary (citable) accession number: A8E5V7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health