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Protein

Guanidinoacetate N-methyltransferase

Gene

gamt

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.UniRule annotation

Pathwayi: creatine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes creatine from L-arginine and glycine.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (gatm)
  2. Guanidinoacetate N-methyltransferase (gamt), Guanidinoacetate N-methyltransferase (gamt)
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18S-adenosyl-L-methionineUniRule annotation1
Binding sitei40SubstrateUniRule annotation1
Binding sitei44SubstrateUniRule annotation1
Binding sitei48S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotation, Transferase
LigandS-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00104; UER00580.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanidinoacetate N-methyltransferaseUniRule annotation (EC:2.1.1.2UniRule annotation)
Gene namesi
Name:gamtImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Organism-specific databases

ZFINiZDB-GENE-051030-97. gamt.

Expressioni

Gene expression databases

BgeeiENSDARG00000070844.
ExpressionAtlasiA8E5I0. baseline and differential.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA8E5I0.
SMRiA8E5I0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 234RMT2InterPro annotationAdd BLAST234

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 72S-adenosyl-L-methionine bindingUniRule annotation6
Regioni88 – 90S-adenosyl-L-methionine bindingUniRule annotation3
Regioni115 – 116S-adenosyl-L-methionine bindingUniRule annotation2
Regioni169 – 170Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG410ISNM. Eukaryota.
ENOG410XPX4. LUCA.
HOVERGENiHBG005801.
KOiK00542.
OMAiRYYAFPQ.
OrthoDBiEOG091G0EPW.
PhylomeDBiA8E5I0.

Family and domain databases

InterProiView protein in InterPro
IPR016550. GuanidinoAc_N-MeTrfase.
IPR026480. RMT2_dom.
IPR029063. SAM-dependent_MTases.
PIRSFiPIRSF009285. GAMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51559. SAM_RMT2. 1 hit.

Sequencei

Sequence statusi: Complete.

A8E5I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAQPIFSK GENCKQVWHD ANADYNAADT HLEIMGKPVM ERWETPYMHS
60 70 80 90 100
LATVAASKGG RVLEIGFGMA IAATKVESFP IEEHWIIECN DGVFQRLQEW
110 120 130 140 150
AKSQPHKVVP LKGLWEEVAP TLPDNHFDGI LYDTYPLSEE TWHTHQFNFI
160 170 180 190 200
KAHAHRMLKP GGVLTYCNLT SWGELLKNKY DNIDKMFQET QVPHLLEAGF
210 220 230
KKEKISTTLM DISPPSECKY YSFNKMITPT IIKE
Length:234
Mass (Da):26,743
Last modified:November 13, 2007 - v1
Checksum:iC998B222A2FA1E1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC153601 mRNA. Translation: AAI53602.1.
RefSeqiNP_001099065.1. NM_001105595.1.
UniGeneiDr.28451.

Genome annotation databases

GeneIDi796865.
KEGGidre:796865.

Similar proteinsi

Entry informationi

Entry nameiA8E5I0_DANRE
AccessioniPrimary (citable) accession number: A8E5I0
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2007
Last sequence update: November 13, 2007
Last modified: September 27, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)