A8E1C4 (DEN_MUHVK) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 9, 2013.
Version 13.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Deneddylase M48 EC=3.4.19.12 EC=3.4.22.- | ||
| Gene names |
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| Organism | Murid herpesvirus 1 (strain K181) (MuHV-1) (Mouse cytomegalovirus) | ||
| Taxonomic identifier | 69156 [NCBI] | ||
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Muromegalovirus ›  | ||
| Virus host | Mus musculus (Mouse) [TaxID: 10090] |
Protein attributes
| Sequence length | 2147 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDKN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity. Ref.3 |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity. |
| Subcellular location | Virion tegument. Host cytoplasm. Host nucleus By similarity. |
| Sequence similarities | Belongs to the herpesviridae large tegument protein family. Contains 1 peptidase C76 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Host-virus interaction Modulation of host ubiquitin pathway by viral deubiquitinase Modulation of host ubiquitin pathway by virus Ubl conjugation pathway |
| Cellular component | Host cytoplasm Host nucleus Virion Virion tegument |
| Domain | Coiled coil |
| Molecular function | Hydrolase Protease Thiol protease |
| Gene Ontology (GO) | |
| Biological_process | modulation by virus of host protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleusInferred from electronic annotation. Source: UniProtKB-SubCell viral tegumentInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 2147 | 2147 | Deneddylase M48 | PRO_0000396954 | |||||
Regions | |||||||||
| Domain | 3 – 223 | 221 | Peptidase C76 | ||||||
| Coiled coil | 649 – 686 | 38 | Potential | ||||||
| Coiled coil | 937 – 964 | 28 | Potential | ||||||
| Coiled coil | 1184 – 1219 | 36 | Potential | ||||||
| Motif | 296 – 303 | 8 | Nuclear localization signal By similarity | ||||||
| Compositional bias | 233 – 238 | 6 | Poly-Gly | ||||||
| Compositional bias | 465 – 468 | 4 | Poly-Thr | ||||||
| Compositional bias | 536 – 539 | 4 | Poly-Ala | ||||||
| Compositional bias | 687 – 690 | 4 | Poly-Ser | ||||||
| Compositional bias | 691 – 694 | 4 | Poly-Pro | ||||||
| Compositional bias | 1567 – 1570 | 4 | Poly-Ser | ||||||
| Compositional bias | 2046 – 2049 | 4 | Poly-Ser | ||||||
Sites | |||||||||
| Active site | 23 | 1 | By similarity | ||||||
| Active site | 156 | 1 | By similarity | ||||||
| Active site | 158 | 1 | By similarity | ||||||
| Site | 10 | 1 | Important for catalytic activity By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Laboratory strains of murine cytomegalovirus are genetically similar to but phenotypically distinct from wild strains of virus." Smith L.M., McWhorter A.R., Masters L.L., Shellam G.R., Redwood A.J. J. Virol. 82:6689-6696(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K181. |
| [2] | "Use of a murine cytomegalovirus K181-derived bacterial artificial chromosome as a vaccine vector for immunocontraception." Redwood A.J., Messerle M., Harvey N.L., Hardy C.M., Kozinowski U.H., Lawson M.A., Shellam G.R. J. Virol. 79:2998-3008(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K181. |
| [3] | "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases." Gastaldello S., Hildebrand S., Faridani O., Callegari S., Palmkvist M., Di Guglielmo C., Masucci M.G. Nat. Cell Biol. 12:351-361(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AM886412 Genomic DNA. Translation: CAP08095.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | A8E1C4. 29 interactions. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR006928. Pept_C76_UL36-USP. [Graphical view] |
| Pfam | PF04843. Herpes_teg_N. 1 hit. [Graphical view] |
| PROSITE | PS51521. HTUSP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DEN_MUHVK | ||||||||
| Accession | Primary (citable) accession number: A8E1C4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |