Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8E1C4

- DEN_MUHVK

UniProt

A8E1C4 - DEN_MUHVK

Protein

Deneddylase

Gene

M48

Organism
Murid herpesvirus 1 (strain K181) (MuHV-1) (Mouse cytomegalovirus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDKN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei10 – 101Important for catalytic activityPROSITE-ProRule annotation
    Active sitei23 – 231PROSITE-ProRule annotation
    Active sitei156 – 1561PROSITE-ProRule annotation
    Active sitei158 – 1581PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Gene namesi
    Name:M48
    OrganismiMurid herpesvirus 1 (strain K181) (MuHV-1) (Mouse cytomegalovirus)
    Taxonomic identifieri69156 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeMuromegalovirus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21472147DeneddylasePRO_0000396954Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Protein-protein interaction databases

    IntActiA8E1C4. 29 interactions.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 223221Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili649 – 68638Sequence AnalysisAdd
    BLAST
    Coiled coili937 – 96428Sequence AnalysisAdd
    BLAST
    Coiled coili1184 – 121936Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi296 – 3038Nuclear localization signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi233 – 2386Poly-Gly
    Compositional biasi465 – 4684Poly-Thr
    Compositional biasi536 – 5394Poly-Ala
    Compositional biasi687 – 6904Poly-Ser
    Compositional biasi691 – 6944Poly-Pro
    Compositional biasi1567 – 15704Poly-Ser
    Compositional biasi2046 – 20494Poly-Ser

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8E1C4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIVRASRDQ SAPVYGPRAG SQCMSNCFTF LHTCYLMGID PVLDTTSLDA     50
    VLDSGARLDA IADEKVKRQA LTDHPYRLGT EIPTVIETPA GITGHALSRP 100
    FNGTAETQDL GGYKCLGILD FLTYARGKPL PVYIIVTVGV HTRGVIVARG 150
    ATYVFDPHTT DLSAEAAVYV CDDFTEAISA LSFFGAMIGD FYYDAVLVYF 200
    TRCRTTLISP SELLVQIMDQ YKDPDIDASV MSGSGGGGPS ISSSAASASA 250
    SVSPLPSGAA SDGVTNGAGA QTSSKSGKKR RAPDVPPQQK GTAKTLRRSR 300
    RPIRLPERLS EVIVEDVQTI EHFRTATQSL PVKPPQEWTM YCGEEPFYRQ 350
    YFVDVGRQLL AHAIDTYVSL DKTTDESAVQ RFESLMGLSD ELDAVIAAAR 400
    ATELSAPPLY KTYLSQRVSA SAVTRTDRLL ASKILALFEE GSATDFETVS 450
    SWLRELLQQL PVENTTTTEA KIAAFVAQHP IPGERAFVCL RNSQVKSLGE 500
    LLSVKRETLK VKYMENDATY RKILDAISKL GLASNAAAAI QSADTSHLDS 550
    EQLASLAQAA EAYAQSAYES CQAKMTELLS TNHNRILTGS LPDSDIAAVL 600
    AAFKAVSENV RALREVELLK SQVHVQLAQL TEDLLYLQTA EVKLEVTPSA 650
    EIKKLRAEYE TARKQISDEE MRIKELIENM EDMITDSSSS PPPPEMLDML 700
    RTQIEEVESM TVDEQDARRA DKVLGKLSTL DEAEAKATEF AQSLSTVNIP 750
    SLNEIKGVKM LKSVLETNAD LRRAYVQAVT GMLENALKQL AEGNLPSDDM 800
    MSRITALAEQ LPTGRQVRAD LLDSTDIVSQ MSRRLRYAAN QKNSTQSLED 850
    VLNFFSENDD MIRKLLKTSW GKPVATVYRR VQVEYDRKME ELRESEWLKR 900
    VKETDIDSPQ TLERLLKTAP NETILAKHAP DMHARLKKRM QSEAEKRTAD 950
    MKRLYEEMRK KVDTDLKVVS DSFSSQTPSM FSSIDLKSCG TSLVRLTKED 1000
    RKAAIATFNA NLTKSLNSLL ASLVTVETAT IAAILKGADP EATSSTGSAG 1050
    GGADRQKHTS TLENNISTLL GWQQKVLLPE TERDLLTIAH LLTALTHMRK 1100
    NWRNPAAAFS STPHTAAYRN FAELANEIES RRTETLARYK SKYAELNASI 1150
    HDNTKANDVT IKPEDTFTAA FRDTVKAMAA PFSTELQARL QARENELKDE 1200
    LSDLNVKLKT KLERYLAKRS SQDARWRDLI TQHRIRLPEG LDVDTNRLRT 1250
    DTVVTLNGII RSAATTLPYI TAKRGLEWTT EFILAAIEEK KDADPGMFAQ 1300
    LATLLDNSQA IARKIEEKIV YNTKVEAEML ETAPEHTRES LSNLQLMLGQ 1350
    LEAKRVVGGE ARYKILSDAI LTKQNKLAFA EDLEKLSARY FELARDIRSS 1400
    KYGLDFDAQL LKTSLLKSEI GQHKKDPPST GEEVGLPEES TTAAKISISS 1450
    LLLGIAALEK YIVAHRTLLD NFVSSQPLIS QAENIPALVG GPGEDDKPGQ 1500
    EPFDWTRIDL SRLSACDVST ALYRGTDVFG ERRVMTARGI QLYLYATHGN 1550
    FVFEAFSHVR GTKGLLSSSS GGGNGSGASS RQKDQQNATV TRRYRSVTVL 1600
    ASIAATLQTF WSEISRYDIR ELLVESGRDP ESAADQRDRR LNTVMNLKLF 1650
    VYVMTVAWSE ATPPVEPGSP EATHALEVSL LDFSTLMAAL HPEYVYAITT 1700
    QPVDATLRGL IARLDRRTVD AAMNTQENPP PYDMRELKAF CLDTKQWTQE 1750
    DIRPQMWMSD LVKQICTSHP RNRDASTSTK LFLYMLATKV LPRDILRCLW 1800
    VQFRPAYASS IASLEELVSA LCDSFFKIYG TTSETVSARL KTGEKVERQV 1850
    VLRHKPTMSL LDEFSQQEAV LDYILGSYVF AIPMTVGIHV TNIVNGRYRL 1900
    IVRHLENLPS DPDFVKVVRS RDLSFDRFGW SYTVQNPVER SWFSLQEDRL 1950
    RHLLTNPPQQ DRTPLVVYDS NTNYAVNAMM PPLKAPPATS RVHLTVKNPF 2000
    STMRMIPHED EGDDAATSET PFTSLPINID FLRRDPPRLK RASGDSSSSV 2050
    PAEDRDPDAR PQDSVPDQSL SEPLFSQTKV SSIVPKDAVT TLSNESISQT 2100
    FQIHPFRALS SAIMAAIEIL QETRLQLDTF ESDMCEAIRR IKILYLH 2147
    Length:2,147
    Mass (Da):238,457
    Last modified:November 13, 2007 - v1
    Checksum:i4C5DB3F99EF1A63A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM886412 Genomic DNA. Translation: CAP08095.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM886412 Genomic DNA. Translation: CAP08095.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi A8E1C4. 29 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Laboratory strains of murine cytomegalovirus are genetically similar to but phenotypically distinct from wild strains of virus."
      Smith L.M., McWhorter A.R., Masters L.L., Shellam G.R., Redwood A.J.
      J. Virol. 82:6689-6696(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K181.
    2. "Use of a murine cytomegalovirus K181-derived bacterial artificial chromosome as a vaccine vector for immunocontraception."
      Redwood A.J., Messerle M., Harvey N.L., Hardy C.M., Kozinowski U.H., Lawson M.A., Shellam G.R.
      J. Virol. 79:2998-3008(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K181.
    3. "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases."
      Gastaldello S., Hildebrand S., Faridani O., Callegari S., Palmkvist M., Di Guglielmo C., Masucci M.G.
      Nat. Cell Biol. 12:351-361(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDEN_MUHVK
    AccessioniPrimary (citable) accession number: A8E1C4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3