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A8E1C4

- DEN_MUHVK

UniProt

A8E1C4 - DEN_MUHVK

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Protein

Deneddylase

Gene
M48
Organism
Murid herpesvirus 1 (strain K181) (MuHV-1) (Mouse cytomegalovirus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDKN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101Important for catalytic activity By similarity
Active sitei23 – 231 By similarity
Active sitei156 – 1561 By similarity
Active sitei158 – 1581 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Name:M48
OrganismiMurid herpesvirus 1 (strain K181) (MuHV-1) (Mouse cytomegalovirus)
Taxonomic identifieri69156 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeMuromegalovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21472147DeneddylasePRO_0000396954Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Protein-protein interaction databases

IntActiA8E1C4. 29 interactions.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 223221Peptidase C76Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili649 – 68638 Reviewed predictionAdd
BLAST
Coiled coili937 – 96428 Reviewed predictionAdd
BLAST
Coiled coili1184 – 121936 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi296 – 3038Nuclear localization signal By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi233 – 2386Poly-Gly
Compositional biasi465 – 4684Poly-Thr
Compositional biasi536 – 5394Poly-Ala
Compositional biasi687 – 6904Poly-Ser
Compositional biasi691 – 6944Poly-Pro
Compositional biasi1567 – 15704Poly-Ser
Compositional biasi2046 – 20494Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8E1C4-1 [UniParc]FASTAAdd to Basket

« Hide

MKIVRASRDQ SAPVYGPRAG SQCMSNCFTF LHTCYLMGID PVLDTTSLDA     50
VLDSGARLDA IADEKVKRQA LTDHPYRLGT EIPTVIETPA GITGHALSRP 100
FNGTAETQDL GGYKCLGILD FLTYARGKPL PVYIIVTVGV HTRGVIVARG 150
ATYVFDPHTT DLSAEAAVYV CDDFTEAISA LSFFGAMIGD FYYDAVLVYF 200
TRCRTTLISP SELLVQIMDQ YKDPDIDASV MSGSGGGGPS ISSSAASASA 250
SVSPLPSGAA SDGVTNGAGA QTSSKSGKKR RAPDVPPQQK GTAKTLRRSR 300
RPIRLPERLS EVIVEDVQTI EHFRTATQSL PVKPPQEWTM YCGEEPFYRQ 350
YFVDVGRQLL AHAIDTYVSL DKTTDESAVQ RFESLMGLSD ELDAVIAAAR 400
ATELSAPPLY KTYLSQRVSA SAVTRTDRLL ASKILALFEE GSATDFETVS 450
SWLRELLQQL PVENTTTTEA KIAAFVAQHP IPGERAFVCL RNSQVKSLGE 500
LLSVKRETLK VKYMENDATY RKILDAISKL GLASNAAAAI QSADTSHLDS 550
EQLASLAQAA EAYAQSAYES CQAKMTELLS TNHNRILTGS LPDSDIAAVL 600
AAFKAVSENV RALREVELLK SQVHVQLAQL TEDLLYLQTA EVKLEVTPSA 650
EIKKLRAEYE TARKQISDEE MRIKELIENM EDMITDSSSS PPPPEMLDML 700
RTQIEEVESM TVDEQDARRA DKVLGKLSTL DEAEAKATEF AQSLSTVNIP 750
SLNEIKGVKM LKSVLETNAD LRRAYVQAVT GMLENALKQL AEGNLPSDDM 800
MSRITALAEQ LPTGRQVRAD LLDSTDIVSQ MSRRLRYAAN QKNSTQSLED 850
VLNFFSENDD MIRKLLKTSW GKPVATVYRR VQVEYDRKME ELRESEWLKR 900
VKETDIDSPQ TLERLLKTAP NETILAKHAP DMHARLKKRM QSEAEKRTAD 950
MKRLYEEMRK KVDTDLKVVS DSFSSQTPSM FSSIDLKSCG TSLVRLTKED 1000
RKAAIATFNA NLTKSLNSLL ASLVTVETAT IAAILKGADP EATSSTGSAG 1050
GGADRQKHTS TLENNISTLL GWQQKVLLPE TERDLLTIAH LLTALTHMRK 1100
NWRNPAAAFS STPHTAAYRN FAELANEIES RRTETLARYK SKYAELNASI 1150
HDNTKANDVT IKPEDTFTAA FRDTVKAMAA PFSTELQARL QARENELKDE 1200
LSDLNVKLKT KLERYLAKRS SQDARWRDLI TQHRIRLPEG LDVDTNRLRT 1250
DTVVTLNGII RSAATTLPYI TAKRGLEWTT EFILAAIEEK KDADPGMFAQ 1300
LATLLDNSQA IARKIEEKIV YNTKVEAEML ETAPEHTRES LSNLQLMLGQ 1350
LEAKRVVGGE ARYKILSDAI LTKQNKLAFA EDLEKLSARY FELARDIRSS 1400
KYGLDFDAQL LKTSLLKSEI GQHKKDPPST GEEVGLPEES TTAAKISISS 1450
LLLGIAALEK YIVAHRTLLD NFVSSQPLIS QAENIPALVG GPGEDDKPGQ 1500
EPFDWTRIDL SRLSACDVST ALYRGTDVFG ERRVMTARGI QLYLYATHGN 1550
FVFEAFSHVR GTKGLLSSSS GGGNGSGASS RQKDQQNATV TRRYRSVTVL 1600
ASIAATLQTF WSEISRYDIR ELLVESGRDP ESAADQRDRR LNTVMNLKLF 1650
VYVMTVAWSE ATPPVEPGSP EATHALEVSL LDFSTLMAAL HPEYVYAITT 1700
QPVDATLRGL IARLDRRTVD AAMNTQENPP PYDMRELKAF CLDTKQWTQE 1750
DIRPQMWMSD LVKQICTSHP RNRDASTSTK LFLYMLATKV LPRDILRCLW 1800
VQFRPAYASS IASLEELVSA LCDSFFKIYG TTSETVSARL KTGEKVERQV 1850
VLRHKPTMSL LDEFSQQEAV LDYILGSYVF AIPMTVGIHV TNIVNGRYRL 1900
IVRHLENLPS DPDFVKVVRS RDLSFDRFGW SYTVQNPVER SWFSLQEDRL 1950
RHLLTNPPQQ DRTPLVVYDS NTNYAVNAMM PPLKAPPATS RVHLTVKNPF 2000
STMRMIPHED EGDDAATSET PFTSLPINID FLRRDPPRLK RASGDSSSSV 2050
PAEDRDPDAR PQDSVPDQSL SEPLFSQTKV SSIVPKDAVT TLSNESISQT 2100
FQIHPFRALS SAIMAAIEIL QETRLQLDTF ESDMCEAIRR IKILYLH 2147
Length:2,147
Mass (Da):238,457
Last modified:November 13, 2007 - v1
Checksum:i4C5DB3F99EF1A63A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM886412 Genomic DNA. Translation: CAP08095.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM886412 Genomic DNA. Translation: CAP08095.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi A8E1C4. 29 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Laboratory strains of murine cytomegalovirus are genetically similar to but phenotypically distinct from wild strains of virus."
    Smith L.M., McWhorter A.R., Masters L.L., Shellam G.R., Redwood A.J.
    J. Virol. 82:6689-6696(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K181.
  2. "Use of a murine cytomegalovirus K181-derived bacterial artificial chromosome as a vaccine vector for immunocontraception."
    Redwood A.J., Messerle M., Harvey N.L., Hardy C.M., Kozinowski U.H., Lawson M.A., Shellam G.R.
    J. Virol. 79:2998-3008(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K181.
  3. "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases."
    Gastaldello S., Hildebrand S., Faridani O., Callegari S., Palmkvist M., Di Guglielmo C., Masucci M.G.
    Nat. Cell Biol. 12:351-361(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDEN_MUHVK
AccessioniPrimary (citable) accession number: A8E1C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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