ID   TARS_RHISY              Reviewed;         771 AA.
AC   A8C981;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   27-MAR-2024, entry version 44.
DE   RecName: Full=Taraxerol synthase;
DE            Short=RsM2;
DE            EC=5.4.99.35;
GN   Name=M2;
OS   Rhizophora stylosa (Bakau) (Red mangrove).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Rhizophoraceae; Rhizophora.
OX   NCBI_TaxID=98588;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=17803686; DOI=10.1111/j.1742-4658.2007.06025.x;
RA   Basyuni M., Oku H., Tsujimoto E., Kinjo K., Baba S., Takara K.;
RT   "Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae.";
RL   FEBS J. 274:5028-5042(2007).
CC   -!- FUNCTION: Multifunctional triterpene synthase producing taraxerol,
CC       beta-amyrin and lupeol in the ratio 70:17:13.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = taraxerol; Xref=Rhea:RHEA:30431,
CC         ChEBI:CHEBI:9402, ChEBI:CHEBI:15441; EC=5.4.99.35;
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB263204; BAF80442.1; -; mRNA.
DR   AlphaFoldDB; A8C981; -.
DR   SMR; A8C981; -.
DR   KEGG; ag:BAF80442; -.
DR   BioCyc; MetaCyc:MONOMER-14454; -.
DR   BRENDA; 5.4.99.35; 5362.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0031559; F:oxidosqualene cyclase activity; IEA:UniProt.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   Gene3D; 1.50.10.20; -; 2.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   NCBIfam; TIGR01787; squalene_cyclas; 1.
DR   PANTHER; PTHR11764:SF58; BETA-AMYRIN SYNTHASE-RELATED; 1.
DR   PANTHER; PTHR11764; TERPENE CYCLASE/MUTASE FAMILY MEMBER; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 2.
PE   2: Evidence at transcript level;
KW   Isomerase; Repeat.
FT   CHAIN           1..771
FT                   /note="Taraxerol synthase"
FT                   /id="PRO_0000412988"
FT   REPEAT          150..191
FT                   /note="PFTB 1"
FT   REPEAT          516..561
FT                   /note="PFTB 2"
FT   REPEAT          642..683
FT                   /note="PFTB 3"
FT   ACT_SITE        487
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   771 AA;  87831 MW;  8515AFA482981022 CRC64;
     MGVWRLKIGE GANNPYLTST NNFVGRQTWV FEPDGGTPEE RDQVEEARQN YFKNRFRVRP
     CSDLLWQMQF LREKNFRQKI PQVKVRDGEE INYETVTNAI RRSAHYLSAT QSSDGFWPAD
     ASAPVFYLAP WVIGLYVIGH LNTVFPAEHQ KEILRYIYCH QNEDGGWGLH YEDGGTMFGT
     AFNYVCMRIL GEGPGGGRDN ACERARKGIL DHGGVTYIPS GGKTWLAMLG VFDWSGCNPM
     PPEFWMLPPF FPMHPAQMWC YCRIVYMPMS YLYGRRFVGP ITPLVQQLRE ELHTQPFHEI
     EWSKARHLCA KEDLFHRRPW IQELFWDCLH TFAEPLLTRW PLNNFIREKA LKITMEHVHY
     DDKASHYINP GSVEKVICMV ACWVEDPSGE PFQRHLARIS DYVWIAEDGM RITGIGSQTW
     DAALSIQALI ACNLIEEMGP TLKKGYDFLK NSQAKDNPPG DFKRMYRHFG KGAWAFSSQD
     YGVIALDCTA ESLMCCLHFS MMPPEIVGEK LEPEKLYLAV DFILSLQSKN GGLTCWEPAR
     GGKWLEVLNP LEFFENIVVE HEYVEVTASA INALVMFKKR YPGYREKEIE HFISKAVHYL
     IQTQFPNGPW YGVWGICFMY GTYFALKGLA AAGNTYANCP AIPKAVDFLL KTQCQDGGWG
     ESYLSGTTKV YTPLEGNRSN LVQTAWALMG LIHSGQAERD PTPLHRSAKL LINSQTSDGD
     FPQQDSTGLL KGSCAMHYAA YRNIFPLWAL AAYRTHVLGL TSKAHSSAVM E
//