ID   GERS_RHISY              Reviewed;         759 AA.
AC   A8C980;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   27-MAR-2024, entry version 49.
DE   RecName: Full=Germanicol synthase;
DE            Short=RsM1;
DE            EC=5.4.99.34;
GN   Name=M1;
OS   Rhizophora stylosa (Bakau) (Red mangrove).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Rhizophoraceae; Rhizophora.
OX   NCBI_TaxID=98588;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   TISSUE=Leaf;
RX   PubMed=17803686; DOI=10.1111/j.1742-4658.2007.06025.x;
RA   Basyuni M., Oku H., Tsujimoto E., Kinjo K., Baba S., Takara K.;
RT   "Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae.";
RL   FEBS J. 274:5028-5042(2007).
CC   -!- FUNCTION: Multifunctional triterpene synthase producing germanicol,
CC       beta-amyrin and lupeol in the ratio 63:33:4.
CC       {ECO:0000269|PubMed:17803686}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = germanicol; Xref=Rhea:RHEA:31003,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:62455; EC=5.4.99.34;
CC         Evidence={ECO:0000269|PubMed:17803686};
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB263203; BAF80441.1; -; mRNA.
DR   AlphaFoldDB; A8C980; -.
DR   SMR; A8C980; -.
DR   KEGG; ag:BAF80441; -.
DR   BioCyc; MetaCyc:MONOMER-14453; -.
DR   BRENDA; 5.4.99.34; 5362.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0042300; F:beta-amyrin synthase activity; IEA:UniProt.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   Gene3D; 1.50.10.20; -; 2.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   NCBIfam; TIGR01787; squalene_cyclas; 1.
DR   PANTHER; PTHR11764:SF58; BETA-AMYRIN SYNTHASE-RELATED; 1.
DR   PANTHER; PTHR11764; TERPENE CYCLASE/MUTASE FAMILY MEMBER; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 2.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..759
FT                   /note="Germanicol synthase"
FT                   /id="PRO_0000412987"
FT   ACT_SITE        485
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   759 AA;  87523 MW;  8F2939D76613A645 CRC64;
     MWRLKIAEGG NDPYLYSTNN YVGRQIWEFD PDAGTPEERA KAEEARQNFY KNRYQVKPSG
     DLLWRLQFLR EKNFKQTIPQ VRIEEGEEIT REKATTALRR AVQFFSALQA SDGHWPAENA
     GPLFFLPPLV MCMCITGHLD TVFPAEHRKE ILRYIYYHQN EDGGWGLHIE GHSTMFCTAL
     NYICMRILGE GPNGGQDDAC TRARKWIHDH GSVTNIPSWG KTWLSILGVY DWSGCNPMPP
     EFWMLPSFLP MHPAKMWCYC RMVYMPMSYL YGKRFVGLIT PLIQQLREEL FTQPYDQINW
     KKNCHQCAPE DLYYPHPFIQ DLIWDCLYIS MEPLLTRWPL NMIIRKKALE LTMKHIHYED
     GSSRYITIGC VEKVLCMLAC WVEDPNGDYF KKHLARIPDY IWVAEDGMKM QSFGSQQWDT
     GFAIQALLAT NLTDEIGGVL RRGHDFIKKS QVQDNPSGDF KSMYRHISKG SWTFSDQDHG
     WQVSDCTAEG LKCCLLFSMM PPEIVGEHME PERLYDSVNV LLSLQSKNGG LSAWEPAGAQ
     DWLELLNPTE FFADIVIEHE YVERTSSAIH ALVLFKKLYP GHRKKEIEDF IAKSVRFLES
     IQTSDGTWYG NWGVCFTYGT WFALGGLAAA GKTYNSCLAM RKAVDFLLRI QKDDGGWGES
     YLSCPEKKYV PLEANHSNLV HTAWAMMALV HAGQMDRDPT PLHRAAKLMI NSQLEDGDFP
     QQEITGVFNR NCMLHYAAYR NIYPLWALAE YCRRVPLPS
//